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MilliporeSigma

IEVH00005

Immobilon® -E PVDF Membrane

1 roll, 27 cm x 1.875 m, 0.45 µm pore size, transfer membrane wets out in transfer buffer or water

Synonym(s):

Western blotting membrane, blotting membrane, transfer membrane

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.23
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination

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Product Name

Immobilon®-E PVDF Membrane, 1 roll, 27 cm x 1.875 m, The first PVDF transfer Membrane for western blotting that wets out in transfer buffer or water. For chemiluminescence or chromogenic detection.

material

PVDF membrane, plain filter, white filter

feature

easily wetted with water

manufacturer/tradename

Immobilon®

technique(s)

western blot: suitable

filter L × W

27 cm × 1.875 m

pore size

0.45 μm pore size

compatibility

for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination

detection method

chemiluminescent, colorimetric, radioactive

shipped in

ambient

Quality Level

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This Item
IEVH07850IEVH09120IEVH85R
Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

detection method

chemiluminescent, radioactive, colorimetric

detection method

chemiluminescent, colorimetric, radioactive

detection method

chemiluminescent, colorimetric, radioactive

detection method

chemiluminescent, colorimetric, radioactive

compatibility

for use with Amido black, for use with Coomassie brilliant blue, for use with Ponceau-S red, for use with Transillumination, for use with Colloidal gold, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with India ink

compatibility

for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination

compatibility

for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination

compatibility

for use with Amido black, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

General description

Primary Binding Mechanisms: Electrostatic, hydrophobic
Filter Code: IEVH
Immobilon-E is the first PVDF membrane for Western blotting that can be wetted with with aqueous transfer buffer. This eliminates the alcohol pre-wet step required with other PVDF membranes.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3


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Articles

Troubleshoot protein transfer issues in Western blotting by understanding causes and optimizing transfer conditions.

Protocols

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

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