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IHCR1005-6

Sigma-Aldrich

Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1

clone LNC1, Chemicon®, from mouse

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eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

LNC1, monoclonal

species reactivity

monkey, lizard, mouse, human, zebrafish, vole, frog, rat, chicken

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TH(7054)
rat ... Th(25085)

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AB9983MAB318-AF555MAB318-AF488
antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified antibody

antibody form

purified antibody

clone

LNC1, monoclonal

clone

polyclonal

clone

LNC1, monoclonal

clone

LNC1, monoclonal

species reactivity

monkey, lizard, mouse, human, zebrafish, vole, frog, rat, chicken

species reactivity

rat, mouse

species reactivity

chicken, zebrafish, mouse, rat, frog, vole, monkey, human

species reactivity

human, chicken, mouse, frog, zebrafish, rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable (paraffin)

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable

General description

Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.

Specificity

Tyrosine Hydroxylase. Recognizes a protein of approximately 59-63 kDa by Western blot. Does not react with the following on Western Blots: dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase, phenethanolamine-N-methyl transferase (PNMT).

Immunogen

Epitope: Recognizes an epitope on the outside of the regulatory N-terminus of tyrosine hydroxylase.
Tyrosine Hydroxylase purified from PC12 cells.

Application

Anti-Tyrosine Hydroxylase Antibody, prediluted, clone LNC1 detects level of Tyrosine Hydroxylase & has been published & validated for use in IH(P).
Antibody is prediluted and ready to use for Immunohistochemistry of formalin-fixed, paraffin-embedded tissues.

Pretreatment: Heat Induced Epitope Retrieval (HIER). Recommend Citrate Buffer, pH 6.0 (Cat. No. 21545). No signal was detected without Epitope retrieval.

Incubation: 30 minutes with IHC Select Detection Kits.

Tyrosine Hydroxylase has been prediluted for use as the primary antibody with Chemicon′s IHC Select Detection Kits and Protocols (Catalog Nos. DAB050, DET-HP1000, APR050, and DET-APR1000), but other supplier′s IHC detection systems may be used. For optimized protocol details, visit www.chemicon.com and select the protocols link under Cat. No.IHCR1005-6.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers

Physical form

Format: Purified
Liquid diluted in PBS, pH 7.2 with stabilizers, 0.2% Tween 20, and 0.1% Kathon as preservative.

Storage and Stability

Maintain at 2-8°C. Refer to vial for expiration dating.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Aquatic Chronic 3 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Thiago B Kirsten et al.
Journal of neuroscience research, 100(7), 1438-1451 (2022-04-02)
The recessive mutant mouse bate palmas (bapa) arose from N-ethyl-N-nitrosourea mutagenesis. Previous studies of our group revealed some behavioral impairments and a mutation in the lysine (K)-specific methyltransferase 2D (Kmt2d) gene. Because mutations in the KMT2D gene in humans are
Tristan Darland et al.
Pharmacology, biochemistry, and behavior, 103(2), 157-167 (2012-08-23)
Finding genetic polymorphisms and mutations linked to addictive behavior can provide important targets for pharmaceutical and therapeutic interventions. Forward genetic approaches in model organisms such as zebrafish provide a potentially powerful avenue for finding new target genes. In order to

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