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IM43

Sigma-Aldrich

Anti-TIMP-3 Mouse mAb (136-13H4)

liquid, clone 136-13H4, Calbiochem®

Synonym(s):

Anti-Tissue Inhibitor of Metalloproteinase-3, Anti-MIG-5

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About This Item

UNSPSC Code:
12352203

biological source

mouse

Quality Level

antibody product type

primary antibodies

clone

136-13H4, monoclonal

form

liquid

contains

≤0.1% sodium azide as preservative

species reactivity

human, baboon, rabbit

should not react with

chicken, porcine, rat, bovine

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TIMP3(7078)

General description

Mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes both the ~27 kDa glycosylated and the ~24 kDa unglycosylated forms of TIMP-3.
Recognizes the ~27 kDa glycosylated and the ~24 kDa non-glycosylated forms of TIMP-3. Does not cross-react with TIMP-1 or TIMP-2 in CaCO-2 and HEK293 cell and eye tissue.
This Anti-TIMP-3 Mouse mAb (136-13H4) is validated for use in Frozen sections, Immunoblotting, Immunofluorescence, Immunoprecipitation, Paraffin sections for the detection of TIMP-3.

Immunogen

Human
a synthetic peptide corresponding to amino acids 170-188 of human TIMP-3

Application


Frozen sections (2-10 g/ml)
Immunoblotting (5 g/ml)
Immunofluorescence (not recommended)
Immunoprecipitation (not recommended)
Paraffin sections (10 g/ml, heat pre-treatment required, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
293 or Caco-2 cells or unfixed eye tissue

Other Notes

Anand-Apte, B., et al. 1997. Invest. Ophthalmol. Vis. Sci.38, 817.
Butler, G.S., et al. 1997. Eur. J. Biochem.244, 653.
Fariss, R.N., et al. 1997. Am. J. Pathol.150, 323.
Vranka, J.A., et al. 1997. Curr. Eye Res.16, 102.
Ruiz, A., et al. 1996. Biochem. Biophys. Res. Commun.226, 467.
Kishnani, N.S., et al. 1995. Matrix Biol.14, 479.
Cottam, D.W. and Rees, R.C. 1993. Intl J. Oncol.2, 861.
Stetler-Stevenson, W. G., et al. 1993. FASEB J.7, 1434.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol. 1(2), 99.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Yasuyuki Shitomi et al.
Molecular biology of the cell, 26(4), 659-673 (2014-12-30)
Discoidin domain receptor 1 (DDR1) is a receptor tyrosine kinase that binds and transmits signals from various collagens in epithelial cells. However, how DDR1-dependent signaling is regulated has not been understood. Here we report that collagen binding induces ADAM10-dependent ectodomain

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