IPVH00010

Millipore

Immobilon-P PVDF Membrane

1 roll, 26.5 cm x 3.75 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

eCl@ss:
32031602
NACRES:
NB.22

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

application(s)

western blot: suitable

filter L × W

26.5 cm × 3.75 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
215 μg/cm2 adsorption capacity (BSA)
294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
radioactive

shipped in

ambient

Related Categories

General description

Immobilon-P transfer membrane is recommended for most Western blotting applications, especially proteins greater than 20 kDa. Immobilon-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Features and Benefits

Immobilon-P PVDF Membrane:
  • Won′t crack, curl or fracture when cut
  • Low background
  • Superior staining capabilities
  • Can be reprobed multiple times

Linkage

Replaces: INCP00010

Other Notes

Primary Binding Mechanisms: Electrostatic, hydrophobic
Filter Code: IPVH

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

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Isabelle Robert et al.
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Articles
Comparison of elution techniques for small-scale protein purification of FLAG® tag proteins using anti-FLAG® M2 magnetic beads.
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Protocols
This page shows and discusses three protocols for stripping and reprobing a western blot membrane.
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