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IPVH304F0

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Immobilon®-P PVDF Membrane

10 sheets, 26 cm x 26 cm, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

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Synonym(s):
Western blotting membrane, blotting membrane, transfer membrane
eCl@ss:
32031602
NACRES:
NB.22

material

PVDF membrane
plain filter
white filter

Quality Level

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

26 cm × 26 cm

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin)
215 μg/cm2 adsorption capacity (BSA)
294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Sypro<TMSYMBOL></TMSYMBOL> ruby
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

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This Item
IPVH07850IPVH00005IEVH08100
feature

hydrophobic

feature

hydrophobic

feature

hydrophobic

feature

easily wetted with water

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

dot blot: suitable, western blot: suitable

technique(s)

western blot: suitable

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

160 μg/cm2 (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

capacity

-

General description

Immobilon®-P PVDF Membrane is an original, and most used, polyvinylidene fluoride (PVDF) transfer membrane for western blotting. It has a 0.45 μm pore size and is recommended for blotting proteins >20 kDa. The Immobilon®-P membrane has excellent protein retention, high physical strength, and broad chemical compatibility, making it ideal for a variety of staining applications and reprobing in immunodetection methods.

Application

Immobilon®-P PVDF Membrane has been used in western blotting.

Features and Benefits

  • Compatible with a variety of detection methods, including radioactive, chromogenic, and chemiluminescent techniques.
  • High protein adsorption and retention ensures greater sensitivity
  • Devoid of crack or curl like nitrocellulose. Can be cut without fracturing and reprobed multiple times.
  • Eliminates the need for blocking and reduces detection times by up to 2 hours.

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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