MAB1096

Anti-Tumor Necrosis Factor-α Antibody, clone 195

clone 195, Chemicon^®, from mouse

• Synonym(s): TNF-alpha
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 195, monoclonal
• species reactivity: human
• should not react with: pig, monkey, canine, mouse, rat, baboon
• packaging: antibody small pack of 25 μg
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; neutralization: suitable; western blot: suitable
• isotype: IgG3
• NCBI accession no.: NM_000594.2
• UniProt accession no.: P01375
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TNF(7124)

Description

Immunogen

Human, recombinant tumor necrosis factor-alpha produced in E. coli.

Application

Research Category
Signaling

Research Sub Category
Growth Factors & Receptors

This Anti-Tumor Necrosis Factor-α Antibody, clone 195 is validated for use in IC, IH, NEUT, WB for the detection of Tumor Necrosis Factor-α.

Western Blot

Immunocytochemistry

Immunohistochemistry

Neutralization: 50 ng of TNF-alpha antibody is required for the neutralization of 1 U TNF-alpha. The TNF-alpha-preparation which is used has a specific activity of 2 x 10(7) U/mg protein [cell lytic assay with L929 cells (mouse transformed fibroblasts) and/or WEHJ 164 cells (mouse fibrosarcoma cells)/actinomycin D].

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

The antibody reacts with human, natural and recombinant TNF-alpha. lt does not react with human TNF-beta (lymphotoxin). lt does not react with TNF-alpha from mouse, rat, canine, porcine, rhesus-monkey or baboon.

SPECIES REACTIVITIES:

Not mouse, rat, canine, porcine, baboon or rhesus-monkey.

Physical form

Format: Purified

Purified immunoglobulin. Liquid in 0.01M PBS pH 7.1, 0.15M NaCl containing no preservatives. Sterile filtered.

Preparation Note

Maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Freeze only once.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1114

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable