MAB1312

Mouse Anti-Human IgG4 Antibody, clone HP6023, Fc

Name: Mouse Anti-Human IgG4 Antibody, clone HP6023, Fc
Brand: MM

clone HP6023, Chemicon^®, from mouse

Synonym(s):

Anti-CD32, Anti-CD32B, Anti-FCG2, Anti-FCGR2, Anti-FCGR2C, Anti-FcGRIIB, Anti-FcRII-c, Anti-FcgammaRIIb, Anti-IGFR2

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.46

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

HP6023, monoclonal

Application:

ELISA, RIA, WB

Citations:

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

secondary antibodies

clone

HP6023, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon^®

technique(s)

ELISA: suitable, radioimmunoassay: suitable, western blot: suitable

isotype

IgG3

NCBI accession no.

NM_001002273.1, NM_001002274.1, NM_001002275.1, NM_004001.3

UniProt accession no.

P31994

shipped in

wet ice

target post-translational modification

unmodified

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This Item	SAB4504023	SAB1405802	SAB4301244
Sigma-Aldrich MAB1312 Mouse Anti-Human IgG4 Antibody, clone HP6023, Fc	Sigma-Aldrich SAB4504023 Anti-phospho-CD32 (pTyr²⁹²) antibody produced in rabbit	Sigma-Aldrich SAB1405802 Anti-FCGR2B antibody produced in mouse	Sigma-Aldrich SAB4301244 Anti-phospho-CD32 (pTyr²⁹²) antibody produced in rabbit
antibody form purified immunoglobulin	antibody form affinity isolated antibody	antibody form purified immunoglobulin	antibody form affinity isolated antibody
biological source mouse	biological source rabbit	biological source mouse	biological source rabbit
technique(s) ELISA: suitable, western blot: suitable, radioimmunoassay: suitable	technique(s) ELISA: 1:1000, western blot: 1:500-1:1000	technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: 1 μg/mL	technique(s) western blot: 1:500-1:1000 (Cell Lysate)
species reactivity human	species reactivity human, rat, mouse	species reactivity human	species reactivity human
isotype IgG3	isotype -	isotype -	isotype IgG
shipped in wet ice	shipped in wet ice	shipped in dry ice	shipped in wet ice

Immunogen

Epitope: Fc fragment

Application

Mouse anti-Human IgG4 Antibody, clone HP6023, Fc is an antibody against Human IgG4 for use in ELISA, RIA & WB.

Research Category
Secondary & Control Antibodies

Research Sub Category
Fragment Specific Secondary Antibodies

This antibody employed as both a capture antibody and a detection antibody for human IgG subclass 4 in enzyme linked immunosorbent assays, radio-immunoassay and EIA affinity immunoblots (Papadea et al., 1985; Hamilton, et al., 1987).

Final working dilutions must be determined by end user.

Biochem/physiol Actions

Human IgG4 Fc. ELISA Specificity: (Hamilton et al., 1987) human IgG subclass 1- 0.08% human IgG subclass 2 - 0.01% human IgG subclass 3 - 0.04% human IgG subclass 4-100% Isoelectric Focusing: mean pI 7.7

Physical form

Format: Purified

Purified immunoglobulin. 0.02MPB+ 0.25M NaCl , Ph=7.6, 0.1% soduim azide.

Preparation Note

Maintain at 2 -8°C for up to one year in undiluted aliquots.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 411493-200UG

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Age-dependent B cell autoimmunity to a myelin surface antigen in pediatric multiple sclerosis.

Katherine A McLaughlin et al.

Journal of immunology (Baltimore, Md. : 1950), 183(6), 4067-4076 (2009-08-19)

Multiple sclerosis (MS) typically manifests in early to mid adulthood, but there is increasing recognition of pediatric-onset MS, aided by improvements in imaging techniques. The immunological mechanisms of disease are largely unexplored in pediatric-onset MS, in part because studies have

Human interleukin 10 induces naive surface immunoglobulin D+ (sIgD+) B cells to secrete IgG1 and IgG3.

F Brière et al.

The Journal of experimental medicine, 179(2), 757-762 (1994-02-01)

During antigen-induced immune responses, human B cells switch isotype from immunoglobulin M (IgM)-IgD to IgG1-4, IgA1-2, or IgE. In the human, no cytokines have yet been demonstrated to act as switch factors for IgG1, IgG2, and IgG3. In this paper

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