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MAB1527

Sigma-Aldrich

Anti-Peripherin Antibody, clone 8G2

culture supernatant, clone 8G2, Chemicon®

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Synonym(s):
Anti-NEF4, Anti-PRPH1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100
300

antibody form

culture supernatant

antibody product type

primary antibodies

clone

8G2, monoclonal

species reactivity

pig, rat, bovine, human, mouse

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

suitability

not suitable for immunohistochemistry (Paraffin)

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... PRPH2(5961)

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This Item
P5117MABN293MABN2395
antibody form

culture supernatant

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

8G2, monoclonal

clone

8G2, monoclonal

clone

6B10.1, monoclonal

clone

2B7, monoclonal

species reactivity

pig, rat, bovine, human, mouse

species reactivity

rat, bovine, pig, mouse, rabbit, feline, human

species reactivity

mouse, rat, human

species reactivity

mouse, bovine

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, indirect ELISA: suitable, microarray: suitable, western blot: 0.5-1.0 μg/mL using total cell extract from rat PC12 cells

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

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General description

Peripherin is a ~57kDa intermediate filament subunit found initially in sensory neurons of the peripheral nervous systems. Subsequently it was found in some sensory and other neurons of the central nervous system and also in PC12 cells. Peripherin is also expressed in certain neuroendocrine tumors and in the insulin producing cells of the pancreas. Peripherin belongs to the Class III family of intermediate filament subunits which includes vimentin, glial fibrillary acidic protein (GFAP) and desmin. Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig′s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors. Autoantibodies to peripherin are frequently seen in the sera of patients with diabetes. Peripherin is not related to peripherin/RDS, a protein of the photoreceptor outer membrane mutations of which are causative of certain forms of slow retinal degeneration.

Specificity

Recognizes Peripherin. Strong staining on rat, mouse, human, pig and cow peripherin. Does not work on chicken or other more distantly related species, which may lack peripherin.

Immunogen

Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990). 57:235-248] containing all but the 4 N-terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6M urea followed by preparative SDS-PAGE

Application

Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB.
Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended.

Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200

Immunoblotting: The antibody is clean and specific for the expected 57kDa band on Western blots.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS

Neuronal & Glial Markers

Target description

57kDa

Physical form

UnPurified mouse culture supernatant containing no preservatives.
Unpurified

Storage and Stability

Maintain for 12 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Rat sensory neurons, rat spinal cord homogenate and peripheral nerve homogenate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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E S Athlan et al.
The Journal of biological chemistry, 272(49), 31073-31078 (1998-01-10)
Formation of protein dimers involving alpha-internexin, peripherin, and the neurofilament (NF) proteins NFH, NFM, and NFL was investigated by partial renaturation of various combinations of individually purified subunits in buffered 2 M urea. Oligomers that were formed were resolved by
Wei Lin et al.
Neuroscience bulletin, 37(9), 1289-1302 (2021-06-03)
Growth differentiation factor 15 (GDF-15) is a member of the transforming growth factor-β superfamily. It is widely distributed in the central and peripheral nervous systems. Whether and how GDF-15 modulates nociceptive signaling remains unclear. Behaviorally, we found that peripheral GDF-15
Leah R Reznikov et al.
American journal of physiology. Lung cellular and molecular physiology, 316(1), L131-L143 (2018-11-09)
Acute airway acidification is a potent stimulus of sensory nerves and occurs commonly with gastroesophageal reflux disease, cystic fibrosis, and asthma. In infants and adults, airway acidification can acutely precipitate asthma-like symptoms, and treatment-resistant asthma can be associated with gastroesophageal
Niliksha Gunewardene et al.
Stem cells international, 2016, 1781202-1781202 (2016-03-12)
Induced pluripotent stem cells (iPSCs) may serve as an autologous source of replacement neurons in the injured cochlea, if they can be successfully differentiated and reconnected with residual elements in the damaged auditory system. Here, we explored the potential of
R C Larivière et al.
Journal of neurochemistry, 81(3), 525-532 (2002-06-18)
Peripherin is a type III intermediate filament (IF) abundantly expressed in developing neurons, but in the adult, it is primarily found in neurons extending to the peripheral nervous system. It has been suggested that peripherin may play a role in

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