MAB1567

Anti-Myelin Associated Glycoprotein Antibody, clone 513

clone 513, Chemicon^®, from mouse

• Synonym(s): MAG
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: 513, monoclonal
• Application: ICC, IHC, WB
• Citations: 98

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 513, monoclonal
• species reactivity: mouse, rat, frog, bovine, chicken, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_002361.2
• UniProt accession no.: P20916
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... MAG(4099)

Description

General description

100 kDa

Myelin-associated glycoprotein (MAG),a member of the immunoglobulin superfamily, is a membrane glycoprotein of 100 kDa, and is thought to be involved in the process of myelination. Axonal regeneration in the adult central nervous system (CNS) is limited by two proteins in myelin, Nogo and MAG. MAG has been implicated in the inhibition of nerve regeneration in the CNS.

Immunogen

L2 epitope carrying glycoprotein extract of 1-2 day old chicken brain.

Application

Detect Myelin Associated Glycoprotein using this Anti-Myelin Associated Glycoprotein Antibody, clone 513 validated for use in IC, IH & WB.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Neurochemistry & Neurotrophins

Western Blot:
20 μg/mL of a previous lot was used on western blot under non-reducing conditions only.{See Ellezam, 2003}

Immunocytochemistry:
5-10 µg/mL of a previous lot was used, methanol fixation required.

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Detects Myelin Associated Glycoprotein (MAG) in immunohistochemistry on frozen sections, immunocytochemistry and Western blot (under non-reducing conditions). MAB1567 is useful for myelinogenesis and multiple sclerosis studies.

Physical form

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 10 mM potassium phosphate buffer, 50 mM NaCl, pH 7.5. Contains no preservative.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
Rat hippocampus tissue, rat brain lysate, PC12 cells.

Evaluated by immunohistochemistry on rat hippocampus.

Immunohistochemistry(paraffin):
Representative image from a previous lot.
Mouse anti-Myelin Associated Glycoprotein [MAG] (Catalog Number MAB1567) localization of MAG in rat hippocampus. Photo courtesy of Dr. Robert Sloviter, University of Arizona.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2