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MAB1585

Sigma-Aldrich

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2

culture supernatant, clone 5A3.2, Chemicon®

Synonym(s):
Anti-ATITHS, Anti-BRN3A, Anti-Oct-T1, Anti-RDC-1, Anti-brn-3A
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

5A3.2, monoclonal

species reactivity

chicken, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... POU4F1(5457)

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This Item
AV38763AB5945SAB1402874
Anti-Brn-3a Antibody Chemicon®, from rabbit

Sigma-Aldrich

AB5945

Anti-Brn-3a Antibody

antibody form

culture supernatant

antibody form

IgG fraction of antiserum

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

clone

5A3.2, monoclonal

clone

polyclonal

clone

polyclonal

clone

7B4, monoclonal

species reactivity

chicken, monkey, rat

species reactivity

guinea pig, rat, human, mouse, rabbit

species reactivity

mouse, human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

Specificity

Recognizes Brn-3a. Shows no reactivity to Brn-3b or Brn-3c by Western blot. Showed no reactivity to Brn-3a knock-out mice.

Immunogen

Amino acids 186-224 of Brn-3a fused to the T7 gene 10 protein (Xiang et al., 1995).
Epitope: POU-domain protein

Application

Anti-Brn-3a Antibody, POU-domain protein, clone 5A3.2 detects level of Brn-3a & has been published & validated for use in IH & WB.
Immunohistochemistry: 1:5-1:125

Western blot: 1:50-1:100

Optimal working dilutions must be determined by end user.

Target description

46 kDa

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Andy J Fischer et al.
The Journal of comparative neurology, 496(4), 479-494 (2006-03-31)
We recently identified large glucagon-expressing neurons that densely ramify neurites in the peripheral edge of the retina and regulate the proliferation of progenitors in the circumferential marginal zone (CMZ) of the postnatal chicken eye (Fischer et al. [2005] J Neurosci
Embryonic retinal cells and support to mature retinal neurons.
Stanke, JJ; Fischer, AJ
Investigative Ophthalmology & Visual Science null
Masahiro Miyake et al.
Nature communications, 6, 6689-6689 (2015-04-01)
Myopia can cause severe visual impairment. Here, we report a two-stage genome-wide association study for three myopia-related traits in 9,804 Japanese individuals, which was extended with trans-ethnic replication in 2,674 Chinese and 2,690 Caucasian individuals. We identify WNT7B as a
Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis.
Badea, TC; Williams, J; Smallwood, P; Shi, M; Motajo, O; Nathans, J
The Journal of Neuroscience null
Sustained ocular hypertension induces dendritic degeneration of mouse retinal ganglion cells that depends on cell type and location.
Feng, L; Zhao, Y; Yoshida, M; Chen, H; Yang, JF; Kim, TS; Cang, J; Troy, JB; Liu, X
Investigative Ophthalmology & Visual Science null

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