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MAB1945

Sigma-Aldrich

Anti-Vitronectin Antibody, clone BV2

clone BV2, Chemicon®, from mouse

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Synonym(s):
Serum spreading factor, S-protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

BV2, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... VTN(7448)

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antibody form

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antibody form

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ascites fluid

antibody form

purified immunoglobulin

clone

BV2, monoclonal

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species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect ELISA: suitable, indirect immunofluorescence: suitable using human cultured fibroblasts, microarray: suitable, western blot: 1:2,500 using a denatured and reduced preparation of purified human plasma vitronectin

technique(s)

ChIP: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Specificity

Human vitronectin. Specificity determined by ELISA and immunoprecipitation of I125 labeled vitronectin purified from plasma (Zanetti et al., 1994). The antibody reacts with vitronectin both in its native and denatured form.

Immunogen

Purified human plasma vitronectin (Preissner, 1985).

Application

Anti-Vitronectin Antibody, clone BV2 is an antibody against Vitronectin for use in ELISA, FC, IP, WB, IH.
ELISA

Immunoprecipitation

Western blot

Immunohistochemistry/immunofluorescence

Inhibits endothelial cell adhesion and spreading on vitronectin-coated surfaces.

Flow Cytometry

Optimal working dilutions must be determined by the end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Physical form

Format: Purified
Purified immunoglobulin by Protein A chromatography. Liquid in PBS containing 0.05% sodium azide and 0.1% BSA.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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