All Photos(2)

MAB1997

Sigma-Aldrich

Anti-Integrin β1 Antibody, clone MB1.2

clone MB1.2, Chemicon®, from rat

Synonym(s):
CD29 antigen, Fibronectin receptor subunit beta, Integrin VLA-4 subunit beta, fibronectin receptor beta subunit, integrin VLA-4 beta subunit, integrin beta 1, integrin beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MB1.2, monoclonal

species reactivity

human, mouse

species reactivity (predicted by homology)

rat

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

application(s)

flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... ITGB1(3688)

General description

Integrin beta-1 (UniProt P05556; also known as CD29, Glycoprotein Iia, GPIIA, Integrin VLA-4 subunit beta, Very late activation protein, beta polypeptide) is encoded by the ITGB1 (also known as FNRB, MDF2, MSK12, VLA-BETA, VLAB) gene (Gene ID 3688) in human. Integrin beta-1 is a 130 kDa transmembrane glycoprotein that interact with various integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptor complexes that bind to specific extracellular matrix proteins. Integrin receptors regulate a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor growth and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to the activation of signal transduction pathways and regulation of gene expression.

Specificity

Reacts with β1 subunit of VLA (β1) integrins

Immunogen

Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132). Mouse Integrin beta-1, as per (Von Ballestrem, C.G., et al. (1996). Hybridoma. 15(2):125-132).

Application

Immunoprecipitation:
A previous lot of this anitbody was used to immunoprecipitate VLA (β1) integrins from lysate of 106 cell equivalent: 2 μg.

Flow cytometry:
A 10 μg/mL concentration of a previous lot was used in FC.

Immunohistochemistry in frozen tissue sections:
20 μg/mL from a previous lot was used. As a result of the wide tissue distribution of VLA (β1), binding of MAB1997 to diverse cell types is commonly observed.
Does not block VLA integrin mediated cell adhesion to matrix proteins. Activation of VLA (β1) integrin signaling properties by MAB1997 has not been characterized.

Western blot:
10 µg/mL. Effective for samples that have been SDS-denatured and heated. Not effective for reduced samples.

Optimal working dilutions must be determined by end user.
Anti-Integrin β1 Antibody, clone MB1.2 is an antibody against Integrin β1 for use in FC, IH, IP & WB.

Quality

Routinely evaluated by Western Blot on C2C12 lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected integrin β1 on 10 μg of C2C12 lysate.

Target description

~88 kDa (non-glycosylated) and ~130 kDa ( glycosylated).

Linkage

Replaces: 04-1109

Physical form

Purified rat monoclonal IgG2κ liquid in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% NaN3
Format: Purified

Analysis Note

Control
Human OVCAR5 cell line whole cell lysate

C2C12 lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificate of Analysis

Certificate of Origin

Dystroglycan does not contribute significantly to kidney development or function, in health or after injury.
Jarad, G; Pippin, JW; Shankland, SJ; Kreidberg, JA; Miner, JH
American Journal of Physiology: Renal Physiology null
?Np63 knockout mice reveal its indispensable role as a master regulator of epithelial development and differentiation.
Romano, RA; Smalley, K; Magraw, C; Serna, VA; Kurita, T; Raghavan, S; Sinha, S
Development null
Distinct roles of the beta 1-class integrins at the developing and the mature hippocampal excitatory synapse.
Huang, Z; Shimazu, K; Woo, NH; Zang, K; Muller, U; Lu, B; Reichardt, LF
The Journal of Neuroscience null
Won-Jing Wang et al.
The Journal of cell biology, 159(1), 169-179 (2002-10-09)
Death-associated protein kinase (DAP-kinase) is a calcium/calmodulin-dependent serine/threonine kinase, and participates in various apoptosis systems. However, its apoptosis-promoting mechanism is poorly understood. Here, we demonstrate that DAP-kinase suppresses integrin-mediated cell adhesion and signal transduction, whereas dominant-negative interference of this kinase...
Reversion of the Jun-induced oncogenic phenotype by enhanced synthesis of sialosyllactosylceramide (GM3 ganglioside).
Miura, Y; Kainuma, M; Jiang, H; Velasco, H; Vogt, PK; Hakomori, S
Proceedings of the National Academy of Sciences of the USA null

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