MilliporeSigma
All Photos(1)

Documents

MAB2223

Sigma-Aldrich

Anti-DNP Antibody, clone 9H8.1

clone 9H8.1, from mouse

Synonym(s):
Dinitrophenol
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

9H8.1, monoclonal

species reactivity (predicted by homology)

all

technique(s)

western blot: suitable

isotype

IgG1κ

shipped in

wet ice

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABN677MABN79MABN1848
antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

9H8.1, monoclonal

clone

5F5.1, monoclonal

clone

8G8.2, monoclonal

clone

8D9, monoclonal

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: suitable

isotype

IgG1κ

isotype

IgG1κ

isotype

IgG1κ

isotype

IgG1κ

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

General description

Dinitrophenol (DNP) is a small water-soluble, hydrophobic ligand that binds to transthyretin at acidic pH and also reduces the formation of amyloid fibril. DNP levels of 100 mg/l or more is greatly toxic to acclimated bacteria. DNP has been associated with possible therapies for neurodegenerative and neurological disorders.

Specificity

Demonstrated to react with a DNP moiety in a wider range of species. This antibody′s reactivity is expected to be species independent.

Immunogen

KLH-conjugated DNP

Application

This Anti-DNP Antibody, clone 9H8.1 is validated for use in WB for the detection of DNP.

Quality

Evaluated by Western Blot in Oxyblot protein standards.

Western Blot Analysis: 0.5 µg/mL of this antibody detected DNP on 10 µg of Oxyblot protein standards.

Target description

Various kDa observed. The Oxyblot protein standards (S7151) were used to test this antibody. These standards are phosphorylase B, BSA, OVA, Carbonic Anhydrase, and trypsin inhibitor which all contain 1-3 DNP residues per protein.

Physical form

Format: Purified

Analysis Note

Control
Oxyblot protein standards

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Jennifer B M Steffen et al.
The Journal of experimental biology, 226(15) (2023-07-20)
Coastal environments commonly experience fluctuations in salinity and hypoxia-reoxygenation (H/R) stress that can negatively affect mitochondrial functions of marine organisms. Although intertidal bivalves are adapted to these conditions, the mechanisms that sustain mitochondrial integrity and function are not well understood.
Lona Zeneyedpour et al.
International journal of molecular sciences, 23(9) (2022-05-15)
We investigated the feasibility of detecting the presence of specific autoantibodies against potential tumor-associated peptide antigens by enriching these antibody-peptide complexes using Melon Gel resin and mass spectrometry. Our goal was to find tumor-associated phospho-sites that trigger immunoreactions and raise
Xiaoding Wang et al.
Cell death and differentiation, 25(12), 2181-2194 (2018-04-19)
Secretory and transmembrane proteins rely on proper function of the secretory pathway for folding, posttranslational modification, assembly, and secretion. Accumulation of misfolded proteins in the endoplasmic reticulum (ER) stimulates the unfolded protein response (UPR), which communicates between the ER and
Ana Reis-Mendes et al.
Pharmaceuticals (Basel, Switzerland), 14(6) (2021-06-03)
Mitoxantrone (MTX) is a pharmaceutical drug used in the treatment of several cancers and refractory multiple sclerosis (MS). Despite its therapeutic value, adverse effects may be severe, namely the frequently reported cardiotoxicity, whose mechanisms need further research. This work aimed
Brígida R Pinho et al.
Free radical biology & medicine, 146, 372-382 (2019-11-22)
Abnormal protein homeostasis (proteostasis), dysfunctional mitochondria, and aberrant redox signalling are often associated in neurodegenerative disorders, such as Huntington's (HD), Alzheimer's and Parkinson's diseases. It remains incompletely understood, however, how changes in redox signalling affect proteostasis mechanisms, including protein degradation

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service