MAB2300

Sigma-Aldrich

Milli-Mark® Pan Neuronal Marker

Milli-Mark Pan Neuronal Marker is an antibody targeting the Pan Neuronal Marker protein, validated for use in ICC, IHC, IF & IHC.

eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

clone

monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Neuro-Chrom

application(s)

immunocytochemistry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable

isotype

IgG1

shipped in

wet ice

General description

Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue. While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs. Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon. To achieve as complete a morphological staining as possible across all parts of neurons, Millipore has developed a monoclonal antibody blend that reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody. This antibody cocktail has been validated in diverse fixations, cell culture and immunohistochemistry protocols, including archival brain tissue, giving researchers a convienent and specific qualitative and quantitative tool for studying neuronal morphology.

Specificity

Reactivity with other species has not been determined.
Cat. # MAB2300 is specific to axons (neurites), dendrites, nucleus, and the cell body of neurons.

Immunogen

Epitope: Whole Neuron Marker

Application

Research Sub Category
Neuronal & Glial Markers
Research Category
Neuroscience

Quality

Routinely tested Rat E18 cortex primary neurons in Immunocytochemistry.

Physical form

Blended monoclonal antibody cocktail in PBS with 0.05% NaN3
Format: Purified

Storage and Stability

Maintain at 2-8°C for up to 1 year from date of receipt.

Analysis Note

Control
Rat E18 cortex primary neurons

Legal Information

MILLI-MARK is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Erdinç Dursun et al.
PloS one, 12(11), e0188605-e0188605 (2017-11-28)
Our recent study indicated that vitamin D and its receptors are important parts of the amyloid processing pathway in neurons. Yet the role of vitamin D receptor (VDR) in amyloid pathogenesis is complex and all regulations over the production of...
Fatal demyelinating disease is induced by monocyte-derived macrophages in the absence of TGF-I? signaling.
Lund, et al.
Nature Immunology, 19, 1-7 (2020)
Short-term fasting induces profound neuronal autophagy.
Alirezaei, M; Kemball, CC; Flynn, CT; Wood, MR; Whitton, JL; Kiosses, WB
Autophagy null
Megan N Evilsizor et al.
Journal of visualized experiments : JoVE, (99), e52293-e52293 (2015-05-21)
Immunohistochemistry is a widely used technique for detecting the presence, location, and relative abundance of antigens in situ. This introductory level protocol describes the reagents, equipment, and techniques required to complete immunohistochemical staining of rodent brain tissue, using markers for...
Erdinç Dursun et al.
Noro psikiyatri arsivi, 51(2), 163-168 (2014-06-01)
Neurodegeneration is a process that is characterized by the loss of neuronal structure and function and eventually ends with neuronal death. An elevated level of inducible nitric oxide synthase (iNOS) is suggested to accompany this process by inducing oxidative and...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service

Social Media

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

MilliporeSigma

Research. Development. Production.

We are a leading supplier to the global Life Science industry with solutions and services for research, biotechnology development and production, and pharmaceutical drug therapy development and production.

© 2021 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved.

Reproduction of any materials from the site is strictly forbidden without permission.