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MAB2503

Sigma-Aldrich

Anti-Elastin Antibody, clone 10B8

clone 10B8, Chemicon®, from mouse

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Synonym(s):
Anti-ADCL1, Anti-SVAS, Anti-WBS, Anti-WS
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

10B8, monoclonal

species reactivity

mouse, bovine, human, chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunofluorescence: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ELN(2006)

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MAB2503

Anti-Elastin Antibody, clone 10B8

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

mouse, bovine, human, chicken

species reactivity

sheep, hamster, bovine, goat, frog, rabbit, porcine, human, guinea pig, canine

species reactivity

human

species reactivity

bovine, pig, human

Gene Information

human ... ELN(2006)

Gene Information

human ... EMILIN3(2006)

Gene Information

human ... FBN1(2200)

Gene Information

human ... FBN1(2200)

clone

10B8, monoclonal

clone

BA-4, monoclonal

clone

26, monoclonal

clone

11C1.3, monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

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Specificity

Recognizes human Elastin. The antibody is also reactive with elastin from chicken, and bovine and mouse. Antibody specificity has been determined by immunoblotting of purified chick tropoelastin and immunoprecipitation of tritium-labeled chick tropoelastin from extracts of chick aorta organ cultures.

Immunogen

Tropoelastin from 17-day old lathyric chick embryo aortae, extracted an purified according to the method of Rich and Foster (1982) Meth. Enzymol. 82: 665-673.

Application

Anti-Elastin Antibody, clone 10B8 detects level of Elastin & has been published & validated for use in ELISA, IF, IP & WB.
Immunoblotting

Immunofluorescence

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

Target description

66 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Skin

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Certificates of Analysis (COA)

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Thomas J Kean et al.
Connective tissue research, 57(4), 319-333 (2016-04-30)
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Stem Cell Reports null
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Endothelial cells (ECs) of the choriocapillaris are one of the first cell types lost during age-related macular degeneration (AMD), and cell replacement therapy is currently a very promising option for patients with advanced AMD. We sought to develop a reliable
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