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MAB2605

Sigma-Aldrich

Anti-ATG5 Antibody, clone 177.19

clone 177.19, from mouse

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Synonym(s):
APG5 autophagy 5-like, APG5 autophagy 5-like (S. cerevisiae), APG5 (autophagy 5, S. cerevisiae)-like, ATG5 autophagy related 5 homolog (S. cerevisiae), ATG5 autophagy related 5-like, Apoptosis-specific protein
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

177.19, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2bκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... ATG5(9474)

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A0731SAB1404726SAB5700062
vibrant-m

MAB2605

Anti-ATG5 Antibody, clone 177.19

species reactivity

human

species reactivity

mouse, human

species reactivity

human

species reactivity

mouse, rat, human

Gene Information

human ... ATG5(9474)

Gene Information

human ... ATG5(9474)
mouse ... Atg5(11793)

Gene Information

human ... ATG5(9474)

Gene Information

human ... ATG5(9474)

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

rabbit

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

100

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General description

ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity

Specificity

Other species have not been tested.
The antibody reacts with the 50 kDa ATG5 protein.

Immunogen

Linear Peptide, aa: 8-318

Application

Immunoprecipitation: HeLa cell lysate
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
This Anti-ATG5 Antibody, clone 177.19 is validated for use in WB, IP for the detection of ATG5.

Quality

Western Blot Analysis: 0.5 ug/mL of this antibody detected ATG5 on HeLa cell lysate.

Target description

50 kDa

Linkage

Replaces: MABC137

Physical form

Format: Purified
Protein G Purified
Purified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Western Blot and Immunoprecipitation: HeLa cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Wen-Tsung Huang et al.
Frontiers in pharmacology, 8, 902-902 (2018-01-13)
Intrinsic or acquired resistance to hormone therapy is frequently reported in estrogen receptor positive (ER+) breast cancer patients. Even though dysregulations of histone deacetylases (HDACs) are known to promote cancer cells survival, the role of different HDACs in the induction
Isabel Pombo Grégoire et al.
PLoS pathogens, 7(12), e1002422-e1002422 (2011-12-17)
Autophagy is a conserved degradative pathway used as a host defense mechanism against intracellular pathogens. However, several viruses can evade or subvert autophagy to insure their own replication. Nevertheless, the molecular details of viral interaction with autophagy remain largely unknown.
Tzu-Yu Lin et al.
Autophagy, 16(7), 1296-1313 (2019-10-16)
BIRC5/Survivin is known as a dual cellular functions protein that directly regulates both apoptosis and mitosis in embryonic cells during embryogenesis and in cancer cells during tumorigenesis and tumor metastasis. However, BIRC5 has seldom been demonstrated as a direct macroautophagy/autophagy

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