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MAB3140

Sigma-Aldrich

Anti-Na+/H+ Exchanger-1 Antibody, CT, clone 4E9

clone 4E9, Chemicon®, from mouse

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Synonym(s):
NHE1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

4E9, monoclonal

species reactivity

amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)

species reactivity (predicted by homology)

mouse, mammals, rat

manufacturer/tradename

Chemicon®

technique(s)

western blot: suitable

isotype

IgG1

suitability

not suitable for immunoprecipitation

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... SLC9A1(6548)

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This Item
AB3081N216MABS1237
antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

-

antibody form

purified immunoglobulin

clone

4E9, monoclonal

clone

polyclonal

clone

C2C12, monoclonal

clone

T4, monoclonal

species reactivity

amphibian, fish, avian, vertebrates (including including fish, amphibians, birds and mammals)

species reactivity

human, rat, bovine, mouse, pig

species reactivity

human, rat, canine, rabbit, guinea pig

species reactivity

gerbil, fish, canine, mouse, human, shark, bovine, avian, ferret, rat, rabbit

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

western blot: suitable

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

immunohistochemistry (frozen sections): 1:200, immunoprecipitation (IP): suitable, western blot: 1:1,000

technique(s)

electron microscopy: suitable, immunocytochemistry: suitable, immunofluorescence: suitable, immunoprecipitation (IP): suitable, western blot: suitable

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General description

Mammalian NHE (Na+/H+ Exchanger) is an integral membrane protein that is known to regulate intracellular pH by removing a proton in exchange for an extracellular sodium ion. There are nine known isofoms of NHE. The best characterized is NHE1, involved not only in intracellular pH control, but cell-volume control, cytoskeletal organization, heart disease and cancer. Transport by the NHE plays a pivotal role in the damage caused to the human myocardium during and following a myocardial infarction, and it is considered to represent a key step in the oncogenic transformation of cancerous cells. NHE comprises an N-terminal membrane domain that transports ions, and a C-terminal cytoplasmic domain that regulates activity and mediates cytoskeletal interactions. NHE2 - NHE5 isoforms are localized to the plasma membrane but exhibit greater tissue-specificity. NH2 and NH3 are more highly expressed in the kidney and intestine. Structurally, a single transmembrane segment from NHE1 has been solved, and the structure of bacterial Na+/H+ antiporter NhaA has been elucidated.

Specificity

Na+/H+ exchanger, isoform NHE1

Immunogen

Epitope: C-terminus
MBP fusion protein containing the entire C-terminal, hydrophylic domain of porcine NHE1.

Application

Anti-Na+/H+ Exchanger-1 Antibody, C-terminus, clone 4E9 detects level of Na+/H+ Exchanger-1 & has been published & validated for use in WB.
Research Category
Neuroscience
Research Sub Category
Ion Channels & Transporters
Works poorly for immunohistochemistry and immunocytochemistry.
Not recommended for immunoprecipitation.

Immunoblotting:
1:1,000 dilution used on a previous lot.

Optimal working dilutions must be determined by the user.

Quality

Routinely evaluated by Western Blot on Human Kidney lysates.

Western Blot:
1:500 dilution of this lot detected Na+/H+ Exchanger-1 on 10 μg of Human Kidney lysates.

Target description

~100-110 kDa

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 liquid in buffer containing 0.02M Phosphate buffer, 0.25M NaCl, pH 7.6 with 0.1% sodium azide

Storage and Stability

Stable for 1 year at 2-8ºC from date of receipt.

Analysis Note

Control
Kidney tissue (basolateral membrane)

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sebastiano Vilella et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 13(4), 207-214 (2003-07-24)
In thyroid cells, the intracellular pH plays a key role in the control of the iodide uptake, since iodide accumulation is associated with an intracellular acidification. In the present paper we studied the kinetic proprieties of the Na(+)/H(+) antiporter (NHE)
C Juel et al.
The Journal of physiology, 548(Pt 2), 639-648 (2003-03-04)
Chronic hypoxia is accompanied by changes in blood and skeletal muscle acid-base control. We hypothesized that the underlying mechanisms include altered protein expression of transport systems and the enzymes involved in lactate, HCO3- and H+ fluxes in skeletal muscle and
Lionel Blanc et al.
American journal of hematology, 90(3), 235-241 (2014-12-18)
Genetic ablation of the ferrireductase STEAP3, also known as TSAP6, leads to severe microcytic and hypochromic red cells with moderate anemia in the mouse. However, the mechanism leading to anemia is poorly understood. Previous results indicate that TSAP6/Steap3 is a
Casper Skovgaard et al.
Journal of applied physiology (Bethesda, Md. : 1985), 122(1), 48-59 (2016-11-20)
The aim of the study was, in runners accustomed to speed endurance training (SET), to examine the effect of increased and maintained frequency of SET on performance and muscular adaptations. After familiarization (FAM) to SET, 18 male (n = 14)
Cian McGinley et al.
Experimental physiology, 101(12), 1565-1580 (2016-10-01)
What is the central question of this study? Following a training intervention, how is the interpretation of adaptations in skeletal muscle H+ transporters influenced by biopsy timing in the context of individual protein and mRNA kinetics after the final exercise

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