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Anti-Vimentin Antibody, clone V9

clone V9, Chemicon®, from mouse


biological source


Quality Level

antibody form

purified antibody

antibody product type

primary antibodies


V9, monoclonal

species reactivity

monkey, feline, pig, bovine, rat, human, chicken, canine

should not react with





immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... VIM(7431)

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This Item
antibody form

purified antibody

antibody form

purified antibody

antibody form

culture supernatant

antibody form

tissue culture supernatant


V9, monoclonal


3CB2, monoclonal


LN-6, monoclonal


SP20, monoclonal

species reactivity

monkey, feline, pig, bovine, rat, human, chicken, canine

species reactivity

chameleon (GLIA 15:389-400 (1995), Prada, F. A., et. al.), rat (GLIA 15:389-400 (1995), Prada, F. A., et. al.), human, lizard

species reactivity

human, mouse

species reactivity

human (tested)










immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable


immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable


immunohistochemistry: suitable (paraffin)


flow cytometry: 1:100, immunoblotting: 1:100, immunohistochemistry: 1:200

General description

Vimentin is the major subunit protein of the intermediate filaments of mesenchymal cells. It is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin has been implicated to be involved in the rate of steroid synthesis via its role as a storage network for steroidogenic cholesterol containing lipid droplets. Immunohistochemical staining for Vimentin is characteristic of sarcomas (of neural, muscle and fibroblast origin) compared to carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin.


Recognizes a 57-60 kDa protein. Shows no cross reactivity with other closely related intermediate filament proteins including desmin and GFAP. In tissue sections, this reagent shows positive reaction on connective tissue, endothelial cells, vascular smooth muscle cells and astrocytes. In tissue culture, typical intermediate filament profiles are seen in fibroblasts and other cell types, which contain vimentin


Purified vimentin from pig eye lens


Anti-Vimentin Antibody, clone V9 is an antibody against Vimentin for use in IC, IH, IH(P) & WB.
A dilution of 1:1000 was used in a previous lot.

5-10 μg/mL was used on a previous lot; can be used on formalin fixed paraffin embedded tissues with traditional high temperature, citrate buffer treatments. Prolonged formalin fixation can reduce antivbody reactivity.

5-10 μg/mL of a previous lot was used in IC.

Optimal working dilutions must be determined by end user.


Evaluated by Western Blot on HeLa lysates.

Western Blot:
1:1000 dilution of this antibody detected VIMENTIN on 10 μg of HeLa lysates

Target description

57-60 kDa


Replaces: MAB3410

Physical form

Format: Purified
Purified mouse monoclonal IgG1 presented in 0.02 M Phosphate buffer, 0.25 M NaCl with 0.1% sodium azide.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids



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Noelia López-Montero et al.
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E Mato et al.
Journal of molecular endocrinology, 52(3), 289-300 (2014-03-20)
Tumor malignancy is associated with the epithelial-mesenchymal transition (EMT) process and resistance to chemotherapy. However, little is known about the relationship between the EMT and the multidrug-resistance gene in thyroid tumor progression. We investigated whether the expression of the ABCG2/BCRP
Shih-Pei Lin et al.
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Hypoxia and serum depletion are common features of solid tumors that occur upon antiangiogenesis, irradiation and chemotherapy across a wide variety of malignancies. Here we show that tumor cells expressing CD133, a marker for colorectal cancer initiating or stem cells
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Prolonged elevations of plasma aldosterone levels are associated with renal pathogenesis. We hypothesized that renal distress could be imposed by an augmented aldosterone-induced protein turnover challenging cellular protein degradation systems of the renal tubular cells. Cellular accumulation of specific protein
Dolores D Mruk et al.
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During spermatogenesis, leptotene spermatocytes residing in the basal compartment of the seminiferous epithelium must traverse the blood-testis barrier (BTB) to gain entry into the adluminal compartment for further development. At the same time, these as well as other germ cell


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