MilliporeSigma
  • MAB3402C3
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MAB3402C3

Sigma-Aldrich

Anti-GFAP Antibody, Cy3 Conjugate

from mouse, CY3 conjugate

Synonym(s):
Glial fibrillary acidic protein, GFAP
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

CY3 conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity

mouse, pig

species reactivity (predicted by homology)

porcine (based on 100% sequence homology), human (based on 100% sequence homology), rat (based on 100% sequence homology), chicken (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... GFAP(2670)

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biological source

mouse

biological source

rabbit

biological source

-

biological source

mouse

conjugate

CY3 conjugate

conjugate

unconjugated

conjugate

-

conjugate

-

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

monoclonal

clone

SP78, monoclonal

clone

RM246, monoclonal, recombinant monoclonal

clone

5G5, monoclonal

species reactivity

mouse, pig

species reactivity

human (tested)

species reactivity

human

species reactivity

human

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

immunohistochemistry: 1:100

technique(s)

immunoblotting: 1:1000-1:3000, immunohistochemistry: 1:200-1:500

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Specificity

The antibody reacts with mouse GFAP. In tissue sections this
antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Immunogen

Purified glial filament protein (Debus, E., 1983).

Application

Anti-GFAP Antibody, Cy3 Conjugate is an antibody against GFAP for use in IC, IH.
Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GFAP in adult mouse brain tissue.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience

Quality

Evaluated by Immunocytochemistry in differentiated ReNcell VMs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GFAP in differentiated ReNcell VMs.

Target description

50 kDa Observed (Using unconjugated antibody MAB3402)

Physical form

Protein A purified
Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Analysis Note

Control
Differentiated ReNcell VMs

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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