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MAB3418

Sigma-Aldrich

Anti-MAP2 Antibody, clone AP20

clone AP20, Chemicon®, from mouse

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Synonym(s):
Microtubule Associated Protein, MAP2A and 2B
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

AP20, monoclonal

species reactivity

bovine, mouse, rat, chicken, quail, Xenopus, human

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

chicken ... Map2(424001)
frog ... Map2(780043)
human ... MAP2(4133)
mouse ... Map2(17756) , Map2(281294)
rat ... Map2(25595)

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M2320M9942M3696
vibrant-m

MAB3418

Anti-MAP2 Antibody, clone AP20

clone

AP20, monoclonal

clone

AP-20, monoclonal

clone

HM-2, monoclonal

clone

polyclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

species reactivity

bovine, mouse, rat, chicken, quail, Xenopus, human

species reactivity

Xenopus, mouse, quail, human, bovine, rat, aquatic salamander

species reactivity

rat, chicken, human, mouse, bovine, quail

species reactivity

rat, mouse, human

isotype

IgG

isotype

IgG1

isotype

IgG1

isotype

-

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General description

MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).

Specificity

Expected to cross-react with human, cow, chicken, quail, and Xenopus.
MAB3418 binds specifically to MAP-2.

Immunogen

Bovine brain microtubule protein.

Application

Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.
Research Category
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Western Blot:
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.

Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).

Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

Optimal working concentrations must be determined by the end user.

Quality

Routinely evaluated by Western Blot on Rat Brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.

Target description

230 kDa

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.

Storage and Stability

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Analysis Note

Control
Brain, Neuronal culture, Human glioblastoma T98G cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Qifang Wu et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 27(2), 522-535 (2012-10-23)
μ-Opioid receptor (MOR) level is directly related to the function of opioid drugs, such as morphine and fentanyl. Although agonist treatment generally does not affect transcription of mor, previous studies suggest that morphine can affect the translation efficiency of MOR
In vivo reversible regulation of dendritic patterning by afferent input in bipolar auditory neurons.
Wang, Y; Rubel, EW
The Journal of Neuroscience null
Two classes of GABAergic neurons in the inferior colliculus.
Ito T, Bishop DC, Oliver DL
The Journal of Neuroscience null
Isoflurane inhibits growth but does not cause cell death in hippocampal neural precursor cells grown in culture.
Sall, JW; Stratmann, G; Leong, J; McKleroy, W; Mason, D; Shenoy, S; Pleasure, SJ; Bickler, PE
Anesthesiology null
A shared vesicular carrier allows synaptic corelease of GABA and glycine.
Wojcik, SM; Katsurabayashi, S; Guillemin, I; Friauf, E; Rosenmund, C; Brose, N; Rhee, JS
Neuron null

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