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MAB343

Sigma-Aldrich

Anti-APP Antibody, APP 643-695 CT fragment, clone 2.F2.19B4

ascites fluid, clone 2.F2.19B4, Chemicon®

Synonym(s):
APP, Jonas clone
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

clone

2.F2.19B4, monoclonal

species reactivity

human, rat

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

Gene Information

human ... APP(351)

Specificity

Reacts with intact full-length Alzheimer precursor protein (APP) and selectively with the cytoplasmic carboxyl fragment of APP 643-695. Epitope has reportedly been mapped in this paper http://www.impub.co.uk/abs/W386.html

Immunogen

Carboxyl fragment of APP 643-695 / Jonas.
Epitope: APP 643-695 C-terminal fragment

Application

Immunohistochemistry on paraffin sections: 10-20 μg/mL * See protocol below.

Western blot: 10 μg/mL

Optimal working dilutions must be determined by end user.

APPLICATION NOTES FOR MAB343

IMMUNOHISTOCHEMISTRY

1) Prepare paraformaldehyde-fixed paraffin sections. Wash twice for 5 min in xylene to deparaffinize. Wash sections for 5 min in a descending series of alcohol solutions (100%, 96%, 90%, 80%, 70%, 50%, 30%).

2) Wash sections 3 times in distilled water.

3) Wash in TBS (50 mM Tris-HCl, 150 mM NaCl, pH 7.6). To block endogenous peroxidase wash with methanol containing 0.6% H2O2 (v/v) and 10 % horse serum (v/v) for 5 min at room temperature.

4) Wash sections for 5 min in TBS.

5) Incubate sections with MAB343 (diluted in TBS containing 10% horse serum (v/v)) overnight at +4°C or for 2 hours at 37°C in a humid chamber.

6) Wash sections 3 times in TBS for 5 min..

7) Detect with standard secondary antibody detection system (PAP, ABC, etc.).

8) Wash sections in TBS.

9) Embed sections and examine.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
This Anti-APP Antibody, APP 643-695 C-terminal fragment, clone 2.F2.19B4 is validated for use in IH(P), WB for the detection of Alzheimer Precursor Protein.

Physical form

Liquid.
Unpurified

Storage and Stability

Maintain lyophilized material at +2–8°C for up to 12 months. After reconstitution maintain frozen at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Brain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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