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MAB3446

Sigma-Aldrich

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9

ascites fluid, clone 2HP-2G9, Chemicon®

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Synonym(s):
HP1a, Antigen p25, Chromobox Protein Homolog 5
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2HP-2G9, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... CBX5(23468)

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MAB3448MABE664MABE656
vibrant-m

MABE656

Anti-HP1γ Antibody, clone 14D3.1

clone

2HP-2G9, monoclonal

clone

1MOD-1A9, monoclonal

clone

5E9.1, monoclonal

clone

14D3.1, monoclonal

antibody form

ascites fluid

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

Gene Information

human ... CBX5(23468)

Gene Information

human ... CBX1(10951)

Gene Information

human ... CBX1(10951)

Gene Information

human ... CBX3(11335)

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

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General description

Chromatin assembly factor-1 (CAF-1) is a multisubunit protein complex that comprises three polypeptide subunits known as p150, p60, and p48. CAF-1 is a nucleosome assembly factor that deposits newly synthesized and acetylated histones H3/H4 into nascent chromatin during DNA replication.

Heterochromatin is characterized as densely coiled chromatin that generally replicates late during S phase, has a low gene density, and contains large blocks of repetitive DNA that is relatively inaccessible to DNA-modifying reagents. In late S phase, p150 directly associates with heterochromatin associated proteins 1 (HP1α, HP1β and HP1γ). As cells prepare for mitosis, CAF-1 p150 and some HP1 progressively dissociate from heterochromatin, coinciding with the phosphorylation of histone H3. The HP1 proteins reassociate with chromatin at the end of mitosis, as histone H3 is dephosphorylated.

Specificity

Reacts with Heterochromatin protein-1 alpha (HP1alpha) between amino acids 67 and 119.

Immunogen

Recombinant mouse HP1alpha.

Application

Anti-Heterochromatin Protein-1 α Antibody, clone 2HP-2G9 is a Mouse Monoclonal Antibody for detection of Heterochromatin Protein-1 α also known as HP1a, Antigen p25, Chromobox Protein Homolog 5 & has been validated in ELISA, IP & WB.
ELISA: 1:500-1:5,000

Western blot: 1:500-1:5,000

Immunoprecipitation: 1:500-1:5,000

Immunohistochemistry/Immunocytochemistry: Not Recommended

Note: for immunocytochemistry and immunohistochemistry we recommend CHEMICON catalog number MAB3584.

Optimal working dilutions must be determined by the end user.

Target description

25 kDa

Physical form

Liquid ascites fluid containing no preservative.

Analysis Note

Control
Positive Control: HeLa cells, 3T3 cells , F9 cells, mouse embryos

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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A role for heterochromatin protein 1? at human telomeres.
Canudas, S; Houghtaling, BR; Bhanot, M; Sasa, G; Savage, SA; Bertuch, AA; Smith, S
Genes & Development null
Aurora-B/AIM-1 regulates the dynamic behavior of HP1alpha at the G2-M transition.
Terada, Y
Molecular Biology of the Cell null
Kate E Keller et al.
Experimental eye research, 171, 164-173 (2018-03-13)
Cultured trabecular meshwork (TM) cells are a valuable model system to study the cellular mechanisms involved in the regulation of conventional outflow resistance and thus intraocular pressure; and their dysfunction resulting in ocular hypertension. In this review, we describe the
Distinctive patterns of epigenetic marks are associated with promoter regions of mouse LINE-1 and LTR retrotransposons.
Rangasamy, D
Mobile DNA null
Andrey Poleshko et al.
Journal of virology, 82(5), 2313-2323 (2007-12-21)
Integrated retroviral DNA is subject to epigenetic gene silencing, resulting in loss of expression of viral genes as well as reporter or therapeutic genes transduced by retroviral vectors. Possible mediators of such silencing include the histone deacetylase (HDAC) family of

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