MAB348

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11

clone 22C11, Chemicon^®, from mouse

• Synonym(s): APP
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 22C11, monoclonal
• species reactivity: rat, fish, human, mouse, canine, pig, monkey
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000484.2; NM_201413.1; NM_201414.1
• UniProt accession no.: P05067
• shipped in: dry ice
• Gene Information: human ... APP(351)

Description

General description

The β/A4 amyloid precursor protein (APP) is a transmembrane glycoprotein and exists in three isoforms. This protein is composed of a single-pass transmembrane domain, a large extracellular N-terminal domain, and a short cytoplasmic C-terminal tail. The APP gene is located on the human chromosome at 21q21.3-q22.

Specificity

Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Immunogen

Epitope: a.a. 66-81 of APP {N-terminus}

Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Application

Anti-APP A4 Antibody, a.a. 66-81 of APP , clone 22C11 has been used in immunohistochemistry and immunoblotting(1:1000).

Biochem/physiol Actions

The β/A4 amyloid precursor protein (APP) undergoes proteolytic cleavage and produces β/A4 peptide. The extracellular deposition of this peptide is observed in brain cortical parenchyma and cerebro-meningeal blood vessels which marks the pathological condition of Alzheimer′s disease. Mutations of the APP gene lead to the early onset of Alzheimer′s disease and hereditary cerebral hemorrhage with amyloidosis.

Target description

110/120/130 kDa

Physical form

Format: Purified

Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.

Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue, brain cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(C): Not applicable