MAB348

Sigma-Aldrich

Anti-APP A4 Antibody, a.a. 66-81 of APP {NT}, clone 22C11

clone 22C11, Chemicon®, from mouse

Synonym(s):
APP
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

22C11, monoclonal

species reactivity

human, fish, canine, monkey, pig, mouse, rat

manufacturer/tradename

Chemicon®

application(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

Gene Information

human ... APP(351)

General description

Deposits of amyloid protein in senile plaques near nerve processes are found in the brains of aged humand and cases of Alzheimer′s Disease. The principle component of this extracellular amyloid is beta A4, a 4 kDa peptide derived from a larger amyloid precursor protein (APP), which is widely expressed in the brain and body. The creation, transport and function of these proteins is currently an active area of research.

Specificity

Reacts with pre-A4. The antibody recognizes amino acids 66-81 of the N-terminus on the pre-A4 molecule (Hilbich et al., 1993). 22C11 recognizes all three isoforms of APP, immature ~110kDa, sAPP ~120kDa, and mature ~130kDa (Hoffmann et al., 2000). The antibody is known to cross react with APLP2 (Slunt, 1994).

Immunogen

Epitope: a.a. 66-81 of APP {N-terminus}
Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein.

Application

Immunofluorescence

Immunohistochemistry: 5-10 μg/mL on 4% paraformaldehyde/15% picric acid perfused and fixed rat spinal cord. 22C11 also works in paraffin embedded tissues but at lower dilutions (1:10-1:20).

Western blot: reducing conditions, 10μg/ml.

Optimal working dilutions and protocols must be determined by the end user.
Research Category
Neuroscience
Anti-APP A4 Antibody, a.a. 66-81 of APP {N-terminus}, clone 22C11 is an antibody against Alzheimer Precursor Protein A4 for use in IF, IH, IH(P) & WB with more than 85 product citations.
Research Sub Category
Neurodegenerative Diseases

Target description

110/120/130 kDa

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Storage and Stability

Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue, brain cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Heme deficiency may be a factor in the mitochondrial and neuronal decay of aging.
Atamna, H; Killilea, DW; Killilea, AN; Ames, BN
Proceedings of the National Academy of Sciences of the USA null
Deletion of Mint proteins decreases amyloid production in transgenic mouse models of Alzheimer's disease.
Ho, A; Liu, X; Sudhof, TC
The Journal of Neuroscience null
Generation and initial characterization of FDD knock in mice.
Giliberto, L; Matsuda, S; Vidal, R; D'Adamio, L
Testing null
Grb2-mediated alteration in the trafficking of AbetaPP: insights from Grb2-AICD interaction.
Raychaudhuri M, Mukhopadhyay D
Journal of Alzheimer'S Disease null
Neuropathy target esterase is required for adult vertebrate axon maintenance.
Read DJ, Li Y, Chao MV, Cavanagh JB, Glynn P
The Journal of Neuroscience null
Protocols
A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.
Read More

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service