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Anti-Dopamine Transporter Antibody, NT, clone DAT-Nt

culture supernatant, clone DAT-Nt, Chemicon®


biological source


Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies


DAT-Nt, monoclonal

species reactivity

rat, human, mouse, monkey




immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable



NCBI accession no.

UniProt accession no.

shipped in

dry ice

Gene Information

human ... SLC6A3(6531)

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This Item
antibody form

culture supernatant

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody


DAT-Nt, monoclonal







species reactivity

rat, human, mouse, monkey

species reactivity

human, monkey

species reactivity

monkey, human

species reactivity

mouse, rat










immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable


immunohistochemistry: suitable, western blot: suitable


immunohistochemistry: suitable, western blot: suitable


western blot: suitable

General description

The transmembrane dopamine transporter (DAT) is located on the presynaptic nerve terminal and is responsible for terminating dopaminergic transmission by transporting dopamine from the synaptic cleft into the dopaminergic neuron (reuptake). Dopaminergic pathways have been strongly implicated in reward and addiction, motivation, alcoholism, ADHD and degenerative motor diseases such as Parkinson′s, Huntington′s and Chorea.


Recognizes Dopamine transporter, N-terminus. Shows no cross reactivity to the closely related serotonin and norepinephrine transporters (Miller, 1997). Immunolocalization of DAT on paraformaldehyde fixed frozen sections of human brain using MAB369 shows dense punctate staining throughout the caudate, putamen and accumbens (Miller, 1997).


Epitope: N-terminus
N-terminus of human dopamine transporter fused to Glutathione S-transferase.


Research Category
Research Sub Category
Ion Channels & Transporters
This Anti-Dopamine Transporter Antibody, N-terminus, clone DAT-Nt is validated for use in IC, IH, WB for the detection of Dopamine Transporter.
Western blot: Recognizes a diffuse band at approximately 70-85 kDa on western blots of extracts (20 ug total protein) from human caudate, putamen, and nucleus accumbens.

Immunohistochemistry: 4% paraformaldehyde fixed tissue (care should be taken not to over-fix tissue); perfusion followed by less than 90 minutes post-fixation, then cryoprotect. Suggested working dilution 1:1,000-1:10,000. If using on rat tissue, absorbed anti-rat secondary antibodies are recommended, and the use of rat PAP systems, or ABC detection will enhance sensitivity.

Immunocytochemistry: 1:5,000 to 1:10,000.

Immunoblotting (not recommended for use on rat)

Optimal working dilutions and protocols must be determined by end user.


This antibody has been used successfully on 30 μm, free floating, 4% paraformaldehyde fixed mouse brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

4) 3 x 10 minute washes in TBS.

5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

6) 3 x 10 minute washes in TBS.

7) ABC Elite (1:200 Vector Labs) in TBS.

8) 2 x 10 minute washes in TBS.

9) 1 x 10 minute wash in phosphate buffer (no saline).

10) DAB reaction with 0.06% NiCl added for intensification.

11) 2 x 10 minute washes in PBS.

12) 1 x 10 minute wash in phosphate buffer (no saline).

Target description

~ 70-85 kDa

Physical form

UnPurified tissue culture supernatant, filtered through a 0.2 μm membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Storage and Stability

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Positive Control: Brain (caudate, putamen, and nucleus accumbens) tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids



Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Weimin C Hong et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 27(8), 2995-3007 (2013-04-25)
The dopamine transporter (DAT) clears the extracellular dopamine released during neurotransmission and is a major target for both therapeutic and addictive psychostimulant amphetamines. Amphetamine exposure or activation of protein kinase C (PKC) by the phorbol ester PMA has been shown
Flotillins regulate membrane mobility of the dopamine transporter but are not required for its protein kinase C dependent endocytosis.
Sorkina, T; Caltagarone, J; Sorkin, A
Traffic null
Methamphetamine increases locomotion and dopamine transporter activity in dopamine d5 receptor-deficient mice.
Hayashizaki, S; Hirai, S; Ito, Y; Honda, Y; Arime, Y; Sora, I; Okado, H; Kodama, T; Takada, M
Testing null
Armando G Salinas et al.
Neuropharmacology, 108, 275-283 (2016-04-03)
The striatum is typically classified according to its major output pathways, which consist of dopamine D1 and D2 receptor-expressing neurons. The striatum is also divided into striosome and matrix compartments, based on the differential expression of a number of proteins
D Tomas et al.
PloS one, 11(4), e0153424-e0153424 (2016-04-15)
The dyskinesia of Parkinson's Disease is most likely due to excess levels of dopamine in the striatum. The mechanism may be due to aberrant synthesis but also, a deficiency or absence of the Dopamine Transporter. In this study we have

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