MAB3775

Sigma-Aldrich

Anti-Cdc20 Antibody, clone AR12

clone AR12, Chemicon®, from mouse

Synonym(s):
Cell Division Cycle Protein 20 Homolog, p55cdc
eCl@ss:
32160702
NACRES:
NA.41

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

AR12, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

application(s)

immunofluorescence: suitable
immunoprecipitation (IP): suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Specificity

Recognizes human Cdc20

Immunogen

Human Cdc20 recombinant protein

Application

Immunofluorescence

Immunoprecipitation

Optimal working dilutions must be determined by end user.
Anti-Cdc20 Antibody, clone AR12 is a Mouse Monoclonal Antibody for detection of Cdc20 also known as Cell Division Cycle Protein 20 Homolog p55cdc & has been validated in IF & IP.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair

Physical form

Purified immunoglobulin. Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide.
Format: Purified

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 12 months from date of receipt.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

L Sironi et al.
The EMBO journal, 20(22), 6371-6382 (2001-11-15)
Mad2 is a key component of the spindle checkpoint, a device that controls the fidelity of chromosome segregation in mitosis. The ability of Mad2 to form oligomers in vitro has been correlated with its ability to block the cell cycle...
James Bancroft et al.
Molecular biology of the cell, 31(21), 2315-2330 (2020-08-07)
Ubiquitin-dependent proteolysis of cyclin B and securin initiates sister chromatid segregation and anaphase. The anaphase-promoting complex/cyclosome and its coactivator CDC20 (APC/CCDC20) form the main ubiquitin E3 ligase for these two proteins. APC/CCDC20 is regulated by CDK1-cyclin B and counteracting PP1...
Mark D Gurden et al.
Oncotarget, 9(28), 19525-19542 (2016-07-18)
Accurate chromosome segregation is dependent on the spindle assembly checkpoint (SAC). In current models, the key direct role of Aurora B in the SAC has been suggested to be to promote rapid kinetochore localisation of MPS1, allowing MPS1 to generate...
Gang Zhang et al.
Nature communications, 8, 15822-15822 (2017-06-13)
Proper segregation of chromosomes depends on a functional spindle assembly checkpoint (SAC) and requires kinetochore localization of the Bub1 and Mad1/Mad2 checkpoint proteins. Several aspects of Mad1/Mad2 kinetochore recruitment in human cells are unclear and in particular the underlying direct...
Mark D Gurden et al.
Cancer research, 75(16), 3340-3354 (2015-07-24)
Acquired resistance to therapy is perhaps the greatest challenge to effective clinical management of cancer. With several inhibitors of the mitotic checkpoint kinase MPS1 in preclinical development, we sought to investigate how resistance against these inhibitors may arise so that...

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