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MAB3804

Sigma-Aldrich

Anti-53BP1 Antibody, clone BP18

ascites fluid, clone BP18, Chemicon®

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Synonym(s):
p53 Binding Protein 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

BP18, monoclonal

species reactivity

mouse, human

manufacturer/tradename

Chemicon®

technique(s)

immunofluorescence: suitable
western blot: suitable

isotype

IgM

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TP53BP1(7158)

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This Item
MAB3802SAB4503017SAB4503016
antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

BP18, monoclonal

clone

BP13, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

mouse, human

species reactivity

human

species reactivity

mouse, human

species reactivity

rat, mouse, human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunofluorescence: suitable, western blot: suitable

technique(s)

immunofluorescence: suitable, western blot: suitable

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:10000, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000

Specificity

53BP1

Immunogen

Recombinant human 53BP1 proteins.

Application

Anti-53BP1 Antibody, clone BP18 is a Mouse Monoclonal Antibody for detection of 53BP1 also known as p53 Binding Protein 1 & has been validated in IF & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western blot. Recognizes a protein of >250 kD. Suggested dilution buffer is TBST. Suggested blocking buffer is 5% non-fat dry milk.

Immunocytochemistry

Immunoprecipitation. Expected size in immunoblot is >250 kD. Suggested lysis buffer is NETN. Suggested capture agent is Protein A + rabbit anti-mouse linker antibody.

Optimal working dilutions must be determined by the end user.

Physical form

Ascites. Liquid.

Storage and Stability

Maintain at -20°C in undiluted aliquots up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Claudia E Rübe et al.
NPJ aging and mechanisms of disease, 7(1), 7-7 (2021-04-03)
Cellular senescence is an irreversible growth arrest that occurs as a result of damaging stimuli, including DNA damage and/or telomere shortening. Here, we investigate histone variant H2A.J as a new biomarker to detect senescent cells during human skin aging. Skin
Dejie Wang et al.
Science advances, 7(25) (2021-06-20)
53BP1 activates nonhomologous end joining (NHEJ) and inhibits homologous recombination (HR) repair of DNA double-strand breaks (DSBs). Dissociation of 53BP1 from DSBs and consequent activation of HR, a less error-prone pathway than NHEJ, helps maintain genome integrity during DNA replication;
Elisa Varela et al.
Nature communications, 7, 11739-11739 (2016-06-03)
Although telomere length is genetically determined, mouse embryonic stem (ES) cells with telomeres of twice the normal size have been generated. Here, we use such ES cells with 'hyper-long' telomeres, which also express green fluorescent protein (GFP), to generate chimaeric
A rare human centenarian variant of SIRT6 enhances genome stability and interaction with Lamin A.
Simon, et al.
The Embo Journal, 41, e110393-e110393 (2023)
Deeksha Munnur et al.
Cell reports, 26(8), 2028-2036 (2019-02-21)
Although poly-ADP-ribosylation (PARylation) of DNA repair factors had been well documented, its role in the repair of DNA double-strand breaks (DSBs) is poorly understood. NR4A nuclear orphan receptors were previously linked to DSB repair; however, their function in the process

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