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  • MAB3834
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MAB3834

Sigma-Aldrich

Anti-Polyhistidine Tag Antibody

Chemicon®, from mouse

All Photos(1)
eCl@ss:
32160702

biological source

mouse

Quality Level

100

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
western blot: suitable

isotype

IgG1

shipped in

wet ice

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biological source

mouse

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rabbit

biological source

mouse

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

clone

monoclonal

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polyclonal

clone

polyclonal

clone

monoclonal

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunocytochemistry: suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

isotype

IgG1

isotype

-

isotype

-

isotype

IgG

Specificity

Reacts with the epitope MRGSHHHHHH which is present in pQE vectors. It is expected that the antibody will also react with proteins expressed from vectors such as PRSET A, B, and C. The antibody should not react with pAC, N, T, A, B, C baculovirus vectors.

Immunogen

His tagged recombinant protein.

Application

Detect Polyhistidine Tag using this Anti-Polyhistidine Tag Antibody validated for use in ELISA & WB.
Research Category
Epitope Tags & General Use
Research Sub Category
Epitope Tags
Western blot: 1:2,000

ELISA

Optimal working dilutions must be determined by end user.

Linkage

Replaces: MAB3112

Physical form

Format: Purified
Purified immunoglobulin. Liquid containing 0.1% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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25G
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