This antibody is provided as crude tissue culture supernatant. The concentration of supernatant formats is not determined due to interference from other proteins with absorbance readings. Generally, supernatants are expected to contain between 10 µg/mL to 50 µg/mL of specific antibody. Conjugation is not recommended because it is not a pure antibody.
MAB386
Anti-Myelin Basic Protein Antibody
CHEMICON®, rat monoclonal, 12
Anti-Myelin Basic Protein Antibody, a.a. 82-87
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Synonym(s):
MBP
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| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| 1 mL | Check Cart for Availability | $544.00 |
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
12, monoclonal
Species reactivity:
human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all
Application:
ELISA, ICC, IHC, RIA, WB
Citations:
327
$544.00
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A recombinant, preservative-free antibody is available for your target. Try ZRB1219
Product Name
Anti-Myelin Basic Protein Antibody, a.a. 82-87, culture supernatant, clone 12, Chemicon®
biological source
rat
Quality Level
antibody form
culture supernatant
antibody product type
primary antibodies
clone
12, monoclonal
species reactivity
human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... MBP(4155)
mouse ... Mbp(17196)
rat ... Mbp(24547)
General description
18-20 kDa
The two major components of CNS myelin are Myelin Basic Protein (MBP) and proteolipid protein (PLP), occuring in roughly equimolar proportions. There are many neurological disorders associated with deficiencies of myelin assembly and structure. For example Multiple Sclerosis is a human disease due to multiple factors but generally characterized by the degradation of the myelin sheath. MBP ususally refers to the 18.5 kDa isoform, which is one of the most abundant proteins of the myelin sheath of adult human and bovine CNS. Genetically, MBP is the product of differential splicing of a single mRNA transcript. In humans there are 4 isoforms, 21.5, 20.2, 18.5, and 17.2 kDa. Thus, MBP are present in multiple isoforms, part of a large family of developmentally expressed, translocatable, and highly post-translationally modifed proteins with multiple binding partners. Structurally, MBP displays a predominately unorganized form with greater than 75% in random coil. Other non-CNS related functions of myelin include insulin induction and glucagon release from pancreatic islets.
Immunogen
Bovine myelin basic protein
Epitope: a.a. 82-87
Application
Anti-Myelin Basic Protein Antibody, a.a. 82-87 is an antibody against Myelin Basic Protein for use in ELISA, IC, IH, RIA & WB.
Immunohistochemistry:
A previous lot of this antibody was used on frozen sections.
ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.
Western Blotting:
Identifies an 18-20kDa band in westerns.
Optimal working dilutions must be determined by end user.
A previous lot of this antibody was used on frozen sections.
ELISA/RIA:
1:1000 dilution of a previous lot of this antibody was used in ELISA/RIA.
Western Blotting:
Identifies an 18-20kDa band in westerns.
Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Neuronal & Glial Markers
Neurochemistry & Neurotrophins
Biochem/physiol Actions
Reacts with MBP from all species tested including human, bovine, sheep, rabbit, mouse, rat, guinea pig and chicken.
Reacts with MBP from all species tested. It reacts weakly with peptides ending in the phe 91 where the 91-92 phe-phe bond is broken. Synthetic peptide 82-99 reacts very well as does intact MBP. Mapped to the region DENPVV.
Physical form
Liquid containing 0.1% sodium azide.
Unpurified
Preparation Note
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Brain tissue
Brain tissue
Evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.
Western Blot Analysis:
1:500 dilution of antibody lot detected four isoforms of MBP on 10 µg of Mouse brain lysates.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
1 of 1
This Item | |||
|---|---|---|---|
| biological source rat | biological source rabbit | biological source rat | biological source rabbit |
| antibody form culture supernatant | antibody form affinity isolated antibody | antibody form tissue culture supernatant | antibody form serum |
| species reactivity human, rat, sheep, mouse, rabbit, chicken, guinea pig, bovine, all | species reactivity rat, human, mouse | species reactivity horse, chicken, human | species reactivity human, rat |
| clone 12, monoclonal | clone polyclonal | clone 14, monoclonal | clone polyclonal |
| Gene Information human ... MBP(4155) | Gene Information human ... MBP(4155) | Gene Information human ... MBP(4155) | Gene Information human ... MBP(4155) |
| technique(s) ELISA: suitable, immunohistochemistry: suitable, western blot: suitable, immunocytochemistry: suitable, radioimmunoassay: suitable | technique(s) flow cytometry: 1:200-1:500, immunohistochemistry: 1:100-1:200, western blot: 1:500-1:1,000 | technique(s) ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable | technique(s) immunohistochemistry: suitable |
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Storage Class
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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The novel BTB/POZ and zinc finger factor Zbtb45 is essential for proper glial differentiation of neural and oligodendrocyte progenitor cells.
Sodersten, E; Lilja, T; Hermanson, O
Cell Cycle null
Xiao Ling Kuai et al.
Stem cell research & therapy, 6, 30-30 (2015-04-19)
Globoid cell leukodystrophy (GLD) is a severe disorder of the central and peripheral nervous system caused by the absence of galactocerebrosidase (GALC) activity. Cell-based therapies are highly promising strategies for GLD. In this study, G-Olig2 mouse embryonic stem cells (ESCs)
An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of Ranvier.
Gasser, A; Ho, TS; Cheng, X; Chang, KJ; Waxman, SG; Rasband, MN; Dib-Hajj, SD
The Journal of Neuroscience null
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB386 | 04053252264856 |
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Hi! I was wondering what the concentration of the antibody is (how many ug/ml)? I need to calculate the volume of primary antibody for a direct conjugation kit. Thanks! Sven
1 answer-
Helpful?
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Is the MAB386 antibody suitable for use with paraffin sections? If so, what would be the recommended antigen retrieval buffer?
1 answer-
The MAB386 antibody has been recommended for IHC on frozen sections, but it has not been tested for IHC on paraffin sections. Therefore, the performance of this antibody on paraffin-embedded samples for immunohistochemistry cannot be guaranteed.
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