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MAB4051

Sigma-Aldrich

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9

clone 9B9, Chemicon®, from mouse

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Synonym(s):
ACE, CD143, DCP1, Peptidyl-dipeptidase A, Peptidase P
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

9B9, monoclonal

species reactivity

hamster, feline, human, monkey, rat

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1

suitability

not suitable for immunohistochemistry (Paraffin)

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... AGT(183)

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AB16982

Anti-Fas Ligand Antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity purified immunoglobulin

antibody form

purified immunoglobulin

Gene Information

human ... AGT(183)

Gene Information

human ... GABRB2(2561)

Gene Information

human ... FAS(355)

Gene Information

human ... ACE(1636)

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

mouse

species reactivity

hamster, feline, human, monkey, rat

species reactivity

mouse, feline, monkey, bovine

species reactivity

human, mouse, rat

species reactivity

human

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

The previously assigned protein identifier P22966 has been merged into P12821. Full details can be found on the UniProt database.

Specificity

Angiotensin-converting enzyme [ACE] (N-terminal), no reactivity to testicular/germinal form of human ACE (90kDa isoform)

Immunogen

Angiotensin-converting enzyme from human lung

Application

Anti-Angiotensin Converting Enzyme Antibody, clone 9B9 detects level of Angiotensin Converting Enzyme & has been published & validated for use in ELISA, IP, WB, IC, IH.
Immunohistochemistry (Danilov et al., 1987; Falkenhahn et al., 1995; Morrell et al., 1995) : 1:1,000 on fresh frozen or 4% paraformaldehyde fixed tissues. Does not work on paraffin embedded tissue.

Immunocytochemistry: 1:100. Reacts with endothelial cell, macrophages (activated) and THP1 cells (monocyte line).

Immunoblotting: 1:100: ~170-180kDa isoform, reducing conditions.

Immunoprecipitation: 1:1,000-1:10,000

ELISA : 1:1,000

Visualization of lung vessels (Danilov et al., 1989; Klibanov et al., 1988; Hiemisch et al., 1993)

Antibody mediated drug delivery into the lung (Danilov et al., 1991, 1994; Muzykantov et al., 1994, 1996)

Estimation of lung vessel injury (Muzykantov et al., 1991; Atochina et al., 1992)

Structure-function of ACE (Danilov et al., 1994)

Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Hormones & Receptors

Physical form

Format: Purified
Purified immunoglobulin. Liquid in PBS, pH 7.4, 150 mM NaCl containing 0.05% sodium azide.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Chiari Kojima et al.
The Journal of pharmacology and experimental therapeutics, 323(3), 855-860 (2007-09-20)
To elucidate an anti-inflammatory role of angiotensin-converting enzyme inhibitors (ACEIs) in cardiovascular disease, we studied the effect of ACEIs in monocyte adhesion to endothelial cells and underlying molecular mechanisms. Treatment of human monocytic THP-1 cells with monocyte chemoattractant protein-1 (MCP-1;
Rosana I Reis et al.
American journal of physiology. Cell physiology, 315(3), C367-C379 (2018-06-07)
Hypertension is a global health problem, and angiotensin I (ANG I)-converting enzyme (ACE) inhibitors are largely used to control this pathology. Recently, it has been shown that ACE can also act as a transducer signal molecule when its inhibitors or
V V Petrov et al.
Journal of the renin-angiotensin-aldosterone system : JRAAS, 1(4), 342-352 (2002-04-23)
Appearance of angiotensin-converting enzyme (ACE) in fibrotic tissue can be the result of the action either of one particular growth factor or of cross-talk between multiple factors. Transforming growth factor-beta1 (TGF-beta(1)) is an effective inducor of the differentiation of cultured

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