MAB4148

Anti-MRP2 Antibody, clone M2 I-4

culture supernatant, clone M2 I-4, Chemicon^®

• Synonym(s): cMOAT
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: M2 I-4, monoclonal
• Species reactivity: human
• Application: ICC, IHC, WB
• Citations: 9

Properties

• biological source: mouse
• Quality Segment: 300
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: M2 I-4, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_000392.2
• UniProt accession no.: Q92887
• shipped in: wet ice
• Gene Information: human ... ABCC2(1244)

Description

Immunogen

Fusion protein of MRP2 containing aa 215-310 of the protein.

Application

This Anti-MRP2 Antibody, clone M2 I-4 is validated for use in WB, IC, IH for the detection of MRP2.

Western blotting: 1:20 - 1:50 with anti-mouse HRP

Immunocytochemistry: 1:20 - 1:50 on acetone fixed cytospin preparations

Immunohistochemistry: 1:20 on acetone fixed frozen tissue sections

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

This antibody reacts with an internal epitope of MRP2, a 190-200 kDa transmembrane protein previously known as the canalicular multi-organic anion transporter (cMOAT). MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. This antibody does not cross-react with the human MDR1, MRP1, MRP3 or MRP5 gene products.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2