MAB4369

Anti-SRF [Serum Response Factor] Antibody, clone 1E1

clone 1E1, from mouse

• Synonym(s): serum response factor (c-fos serum response element-binding transcription factor)
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1E1, monoclonal
• species reactivity: mouse, human
• technique(s): immunocytochemistry: suitable; western blot: suitable
• input: sample type neural stem cell(s); sample type mesenchymal stem cell(s)
• isotype: IgG1κ
• UniProt accession no.: P11831
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ELK3(2004)

Description

General description

Serum response factor (SRF) is a nuclear transcription factor that binds to the serum response element (SRE), and is essential for embryonic development of the mesoderm, as well as for the maintenance of adult muscle cells and neurons. As this protein is involved in the downstream regulation of many immediate-early genes, it participates in a variety of cell functions, including cell cycle, apoptosis, cell growth, and cell differentiation. SRF is most commonly activated by serum, LPA, LPS, TPA, growth factors, including TNFalpha, as well as antioxidants and UV light.

~52 kDa

Immunogen

Synthetic human SRF peptide, a.a. 406-508.

Application

Detect SRF [Serum Response Factor] using this Anti-SRF [Serum Response Factor] Antibody, clone 1E1 validated for use in WB & IC.

Immunocytochemistry: Recommended working dilution is 1:500.

Research Category
Stem Cell Research

Research Sub Category
Mesenchymal Stem Cells

Neural Stem Cells

Biochem/physiol Actions

A weak interaction with mouse has also been observed. Reactivity with other species has not been tested.

This monoclonal antibody recognizes SRF.

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. Avoid repeat freeze/thaw cycles.

Analysis Note

(Based upon representative data from a previous lot).

Western Blotting: Recommended working dilution is 0.5 µg/mL.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable