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MAB4444

Sigma-Aldrich

Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8

clone mab 8, from mouse

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Synonym(s):
Adenocarcinoma-associated antigen, CD326 antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, KS 1/4 antigen, Major gastrointestinal tumor-associated protein GA733-2, antigen identified by monoclonal antibo
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

clone

mab 8, monoclonal

species reactivity

human

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

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MABT1587SAB4200690ZRB1215
antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

culture supernatant

antibody form

purified antibody

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit (recombinant)

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

shipped in

wet ice

shipped in

dry ice

shipped in

dry ice

shipped in

ambient

UniProt accession no.

P16422

UniProt accession no.

P16422

UniProt accession no.

P16422

UniProt accession no.

P16422

General description

Epithelial Call Adhesion Molecule (EpCAM, CD326) is a transmembrane glycoprotein of approximately 40 kDa. EpCAM was initially identified as a tumor associated marker having high expression in quickly proliferating epithelial tumors. As such, EpCAM has been of significant interest as a means for tumor diagnosis and therapy. EpCAM is also expressed in normal epithelial cells, but in comparatively lower levels. Preliminary studies limited EpCAM’s function to cell-cell adhesion; however more recent studies indicate it to be involved in diverse processes that include cell migration, cell signaling, differentiation and proliferation.

Specificity

This antibody recognizes epithelial cell adhesion molecule (EpCAM), specifically on undifferentiated human embryonic stem cells.

Immunogen

Epitope: Unknown
HES-3 Cells (Choo, et al. (2008) Stem Cells. Jun;26(6):1454-63)

Application

Flow Cytometry Analysis: 0.5 µg from a previous lot detected Epithelial cell adhesion molecule in human embryonic stem cells.

Western Blot Analysis: A previous lot was used by an independent laboratory in WB. (Ng, V., et al., 2010)
Research Category
Stem Cell Research
Research Sub Category
Pluripotent & Early Differentiation

Adhesion (CAMs)
This Anti-Epithelial Cell Adhesion Molecule (EpCAM) Antibody, clone 8 is validated for use in FC, IC, WB for the detection of Epithelial Cell Adhesion Molecule (EpCAM).

Quality

Evaluated by Immunocytochemistry in human embryonic stem cells.

Immunocytochemistry Analysis: 1-10 µg/mL from a previous lot detected epithelial cell adhesion molecule (EpCAM) in human embryonic stem cells.

Target description

~ 40 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human embryonic stem cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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