MAB5258
Anti-Synaptophysin Antibody, clone SY38
clone SY38, Chemicon®, from mouse
Synonym(s):
synaptophysin, Synaptophysin
About This Item
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SY38, monoclonal
species reactivity
bovine, rat, avian, fish, mouse, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... SYP(6855)
General description
Specificity
Immunogen
Application
A previous lot of this antibody was used in Flow Cytometry.
Western Blot:
(Provoda, 2000) Reacts with a 38 kDa transmembrane glycoprotein
Immunohistochemistry:
For relatively cytoplasm-rich neuroendocrinic tumors a final concentration of 1 µg/mL is recommended. Concerning cytoplasm deficient tumors a concentration of 2 µg/mL should be used. Ideal frozen sections (4-5 mm) are obtained from shock-frozen tissue samples. The frozen sections are air-dried and then fixed with acetone for 5-10 min at -20°C. Excess acetone is allowed to evaporate at room temperature. Material fixed in alcohol or formalin and embedded in paraffin can also be used.
It is advantageous to block unspecific binding sites by overlaying the sections with fetal calf serum for 20-30 min at room temperature. Excess of fetal calf serum is removed by decanting before application of the anti-body solution.
Cytocentrifuge preparations of single cells or cell smears are also fixed in acetone. These preparations should but should be followed directly by antibody treatment.
Optimal working dilutions must be determined by end user.
Neuroscience
Synapse & Synaptic Biology
Quality
Western Blot Analysis:
1:1000 dilution of this lot detected synaptophysin on 10 μg of mouse brain lysates.
Target description
Linkage
Physical form
Storage and Stability
Analysis Note
Positive Control: Pancreas tissue
Negative Control: Normal mouse serum
Rat hippocampus tissue, mouse brain lysate.
Other Notes
Legal Information
Disclaimer
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recommended
Storage Class
10 - Combustible liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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