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MAB5316

Sigma-Aldrich

Anti-Rhodopsin Antibody, clone RET-P1

clone RET-P1, Chemicon®, from mouse

Synonym(s):
Opsin 2
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

RET-P1, monoclonal

species reactivity

human, mouse, rat

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... RHO(6010)

Specificity

Reacts with a protein of 39 kDa identified as rhodopsin (opsin). MAB5316 specifically labels the axons and synaptic pedicles of the rods.

CELLULAR LOCALIZATION:

Cytoplasmic

Immunogen

Membrane preparation from adult rat retina.

Application

Detect Rhodopsin using this Anti-Rhodopsin Antibody, clone RET-P1 validated for use in IC & WB.
Immunoblotting: 1 μg/mL

Immunocytochemistry

Immunohistochemistry (frozen and formalin/paraffin): 1-2 μg/mL. Staining of formalin fixed tissue sections requires boiling the tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes.

Optimal working dilutions must be determined by end user.
Research Category
Neuroscience
Research Sub Category
Sensory & PNS

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 10 mM PBS, pH 7.4 with 0.2% BSA and 15 mM sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 6 months.

Analysis Note

Control
POSITIVE CONTROL:

IMR-5 cells, brain or retina.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Quality

Enter Lot Number to search for Certificate of Quality (COQ).

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