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MAB5448

Sigma-Aldrich

Anti-Neurofilament H (200 kDa) Antibody

CHEMICON®, rat monoclonal, TA51

Synonym(s):

NF-H

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1 ML
$498.00

About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

$498.00


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A recombinant, preservative-free antibody is available for your target. Try ZRB1347

Request a Bulk Order

Product Name

Anti-Neurofilament NF-H Antibody, clone TA51, culture supernatant, clone TA51, Chemicon®

biological source

rat

Quality Level

antibody form

culture supernatant

antibody product type

primary antibodies

clone

TA51, monoclonal

species reactivity

human, rat, hamster, monkey

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NEFH(4744)
rat ... Nefh(24587)
rhesus monkey ... Nefh(717705)

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This Item
AB1989AB1991MAB5256A5
Gene Information

human ... NEFH(4744)
rat ... Nefh(24587)
rhesus monkey ... Nefh(717705)

Gene Information

bovine ... Nefh(528842)
human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

Gene Information

human ... NEFH(4744)

Gene Information

human ... NEFH(4744)
mouse ... Nefh(380684)
pig ... Nefh(100156492)
rat ... Nefh(24587)

clone

TA51, monoclonal

clone

polyclonal

clone

polyclonal

clone

NE14, monoclonal

biological source

rat

biological source

rabbit

biological source

rabbit

biological source

mouse

species reactivity

human, rat, hamster, monkey

species reactivity

bovine, rat, human, pig, mouse

species reactivity

avian, reptile

species reactivity

mouse, rat, pig

antibody form

culture supernatant

antibody form

serum

antibody form

serum

antibody form

purified antibody

UniProt accession no.

P12036

UniProt accession no.

P12036

UniProt accession no.

P12036

UniProt accession no.

P12036

Specificity

Neurofilament H (NF-H), carboxy terminal (phosphate dependent (+). Clone is specific for phosphorylated NF-H {Carden et al (1987) 7(11):3489}.

Immunogen

Epitope: carboxy terminal phosphorylation dependent, within the multiphosphorylation repeat in NFH

Application

Anti-Neurofilament NF-H Antibody, clone TA51 is an antibody against Neurofilament NF-H for use in ELISA, IP, WB, IH.
Research Category
Neuroscience
Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers
Western blot: Identifies ~ 200kDa band in reduced samples. Use Tris based buffers for best results as epitope is phosphate dependent; Different regions of the CNS were dissected out immediately and homogenized in BUST buffer (8 M urea, 0.5% SDS, 2% 13-mercaptoethanol, and 50 mM Tris, pH 7.4) at a ratio of i ml/100 mg wet tissue. The tissue suspensions were sonicated well and centrifuged in a TL-100 ultracentrifuge (Beckman Instruments, Fullerton, CA) at 100,000 g

for 1 h at 25°C, and the protein concentrations in the supernatants were determined. Proteins were separated on 7.5% SDS-PAGE gels, {Tu. P et al (1995) J Cell Biol 129:1629-1640}

Immunohistochemistry on frozen sections; not effective on paraffin tissues in original work. {Carden et al, 1987}.

Optimal working dilutions must be determined by end user.

Physical form

Unpurified tissue culture supernatant from a perfusion system, filtered through a 0.2μ membrane prior to vialing. Product contains 20%FBS and Ciprofloxacin at final concentration of 10μg/mL.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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