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Key Documents

MAB5460

Sigma-Aldrich

Anti-Nkx2.1 Antibody, clone 8G7-G3-1

ascites fluid, clone 8G7-G3-1, Chemicon®

Synonym(s):

TTF-1, Thyroid Transcription Factor-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Pricing and availability is not currently available.

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

8G7-G3-1, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μL

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1

NCBI accession no.

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NKX2-1(7080)

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This Item
SAB1404472SAB3500757SAB5500187
biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

Gene Information

human ... NKX2-1(7080)

Gene Information

human ... NKX2-1(7080)

Gene Information

human ... NKX2-1(7080)

Gene Information

human ... NKX2-1(7080)

clone

8G7-G3-1, monoclonal

clone

2F6, monoclonal

clone

-

clone

SP141, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human, rat, mouse

species reactivity

human (tested)

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

ELISA: suitable, western blot: suitable

technique(s)

flow cytometry: 1:100, immunohistochemistry: 1:100

Specificity

Nkx2.1 (Thyroid Transcription Factor-1, TTF-1).

Immunogen

Recombinant rat Nkx2.1.

Application

This Anti-Nkx2.1 Antibody is validated for use in WB, IH(P) for the detection of Nkx2.1.
Western blot. The antibody reacts with a 40 kDa band in immunoblots of nuclear extracts or whole cell lysates from the Nkx2.1 positive cell lines MLE15, H441-4, H345 and rat type II pmeumocyte cells.

Immunohistochemistry. The antibody can be used on formalin-fixed paraffin embedded tissue. Heat induced epitope retrieval is recommended.

Optimal working dilutions must be determined by end user.

Quality

Tested

Other Notes

Please refer to lot specific datasheet.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class

10 - Combustible liquids

wgk_germany

WGK 2


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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