MAB80138 anti hMPV antibody was used in Immunofluorescence in the following study. A549 or WI-38 cells were fixed in PBS containing 4% paraformaldehyde for 10 minutes on ice, followed by permeabilization with 0.1% saponin for 30 minutes and blocking with PBS containing 10% fetal bovine serum and 2.5% bovine serum albumin for 60 minutes at room temperature. The primary antibody against hMPV nucleoprotein (N protein) was incubated overnight at 4°C, followed by secondary antibody GAM 647 for 30 minutes at room temperature. Nuclear staining was achieved with DAPI staining for 10 minutes. The study can be accessed at https://pmc.ncbi.nlm.nih.gov/articles/PMC5772477/.
This product is usually provided at a concentration of 1 mg/mL, and the lot-specific Certificates of Analysis can be found on the website: https://www.sigmaaldrich.com/product/Anti-Human-Metapneumovirus-Antibody-clone-507,MM_NF-MAB80138#anchor_COA
MAB80138
Anti-Human Metapneumovirus Antibody, clone 507
clone 507, Chemicon®, from mouse
Synonym(s):
hMPV
Select a Size
| Pack Size | SKU | Availability | Price |
|---|
About This Item
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
507, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, western blot: suitable
isotype
IgG2a
shipped in
wet ice
General description
Application
Biochem/physiol Actions
Physical form
Other Notes
Legal Information
1 of 1
This Item | |||
|---|---|---|---|
| antibody form purified antibody | antibody form purified antibody | antibody form purified antibody | antibody form purified antibody |
| species reactivity human | species reactivity human | species reactivity human | species reactivity human |
| clone 507, monoclonal | clone 406, monoclonal | clone 67, monoclonal | clone 134, monoclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source mouse |
| technique(s) ELISA: suitable, immunofluorescence: suitable, flow cytometry: suitable, western blot: suitable | technique(s) ELISA: suitable, flow cytometry: suitable | technique(s) ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable | technique(s) ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable |
| shipped in wet ice | shipped in wet ice | shipped in wet ice | shipped in wet ice |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Related Content
Global Trade Item Number
| SKU | GTIN |
|---|---|
| MAB80138 | 04053252725272 |
| MAB80138-K | 04053252358951 |
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Regarding MAB80138, anti hMPV antibody, is there any paper that was published using this antibody for Immunofluorescence? In addition was is the approximate concentration of the antibody that you will send?
1 answer-
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