MAB8560

Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4

ascites fluid, clone 583-G8-G2-A4, Chemicon^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: 583-G8-G2-A4, monoclonal
• Species reactivity: human
• Application: IF
• Citations: 2

Properties

• biological source: mouse
• Quality Segment: 300
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: 583-G8-G2-A4, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable
• isotype: IgG1
• shipped in: wet ice

Description

Application

Detect Poliovirus 1 using this Anti-Poliovirus 1 Antibody, clone 583-G8-G2-A4 validated for use in IF.

IFA at 1:100-1:400.

Optimal working dilutions must be determined by end user.

SUGGESTED IFA

PROTOCOL:

Substrate slides:

1. Scrape and spot cells onto microscope slide wells.

2. Air Dry.

3. Fix in cold acetone for 10 minutes at +4°C.

Stain:

1. Add 15 μL diluted polio antibody.

2. Incubate at +37°C for 30 minutes in a humid chamber.

3. Rinse in PBS for 5 minutes; repeat with fresh PBS.

4. Add 15 μL diluted labeled antibody.

5. Repeat steps 2 and 3

6. Mount with buffered glycerol.

Research Category
Infectious Diseases

Research Sub Category
Infectious Diseases - Viral

Biochem/physiol Actions

Reacts with the Poliovirus 1.

Preparation Note

Store at -20°C for up to 12 months in convenient aliquots. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable