MilliporeSigma
All Photos(2)

Documents

MABC117

Sigma-Aldrich

Anti-Podoplanin Antibody, clone NZ1.2

clone NZ1.2, from rat

Sign Into View Organizational & Contract Pricing

Synonym(s):
Aggrus, Glycoprotein 36, Gp36, PA2.26 antigen, T1-alpha, T1A
eCl@ss:
32160702
NACRES:
NA.41

biological source

rat

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

NZ1.2, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... PDPN(10630)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
MABT855MMABT853MMABT852M
antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

NZ1.2, monoclonal

clone

LpMab-13, monoclonal

clone

LpMab-19, monoclonal

clone

LpMab-21, monoclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable (paraffin)

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable (paraffin)

isotype

IgG2aκ

isotype

IgG1κ

isotype

IgG2bκ

isotype

IgG2aκ

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Human podoplanin (T1alpha-2, aggrus and gp36) is a 38 kDa type-1 transmembrane glycoprotein that consists of 162 amino acids, nine of which form the intracellular domain. The highly O-glycosylated extracellular domain contains a sialic acid that is linked to an alpha-2,3 galactose, which forms the main part of the protein’s carbohydrate moieties. It is expressed in a select variety of human tissues and organs, and is also found in lymphatic, but not blood vessel endothelium. While its mode of action remains unclear, podoplanin is suggested to play a critical role in several physiological processes and conditions such as cell migration, cytoskeleton organization, tissue development, and tissue repair, as well as in carcinogenesis and tumor cell invasion.

Specificity

This antibody detects Podoplanin at the PLAG domain.

Immunogen

Epitope: PLAG Domain
Protein sequence corresponding to the PLAG domain of human Podoplanin.

Application

Anti-Podoplanin Antibody, clone NZ1.2 is an antibody against Podoplanin for use in WB, IH.
Immunohistochemistry Analysis: A 1:300 dilution from a representative lot detected Podoplanin in human lung adenocarcinoma and colorectal tissue.
Research Category
Apoptosis & Cancer
Research Sub Category
Cytoskeleton

Quality

Evaluated by Western Blot in human lung tissue lysate.

Western Blot Analysis: A 1:2,000 dilution of this antibody detected Podoplanin in 10 µg of human lung tissue lysate.

Target description

~25 kDa observed.
4 isoforms are produced by alternative splicing, at molecular weights of ~17 kDa (isoform 1), ~18 kDa (isoform 2), ~25 kDa (isoform 3), and ~25 kDa (isoform 4).

Physical form

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human lung tissue lysate.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service