MABC1171

Anti-ALK Antibody, clone ALK1

clone ALK1, from mouse

• Synonym(s): ALK tyrosine kinase receptor, EC: 2.7.10.1, Anaplastic lymphoma kinase, CD246
• eCl@ss: 32160702

Properties

• biological source: mouse
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: ALK1, monoclonal
• species reactivity: human
• packaging: antibody small pack of 25 μg
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG3κ
• NCBI accession no.: NP_004295
• UniProt accession no.: Q9UM73
• Gene Information: human ... ALK(238)

Description

General description

ALK tyrosine kinase receptor (UniProt: Q9UM73; also known as EC: 2.7.10.1, Anaplastic lymphoma kinase, CD246) is encoded by the ALK gene (Gene ID: 238) in human. ALK tyrosine kinase receptor is a single-pass type I membrane protein that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the development and function of the nervous system. It is predominantly expressed in the central nervous system, intestine, and testis. It acts as a receptor for ligands pleiotrophin (PTN) and midkine (MDK). It is activated and homodimerizes by ligand-binding and subsequent phosphorylation. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. It is inactivated through dephosphorylation by receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) in the absence of any stimulation by a ligand. A chromosomal aberration involving ALK has been reported in non-Hodgkin lymphoma where the kinase is constitutively activated. Another chromosomal aberration involving ALK has also been linked with anaplastic large-cell lymphoma (ALCL). ALK tyrosine kinase receptor is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off in the mature form. ALK tyrosine kinase receptor consists of an extracellular domain (aa 19-1038); a transmembrane domain (aa 1039-1059); and a cytoplasmic domain (aa 1060-1620).

Specificity

Clone ALK1 specifically detects human ALK tyrosine kinase receptor. It targets an epitope with in 102 amino acids from the C-terminal region.

Immunogen

His-tagged full length human recombinant ALK tyrosine kinase receptor.

Application

Anti-ALK, clone ALK1, Cat. No. MABC1171, is a mouse monoclonal antibody that detects ALK tyrosine kinase receptor and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Research Category
Signaling

Western Blotting Analysis: A representative lot detected ALK in Western Blotting applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

Immunohistochemistry Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

Immunofluorescence Analysis: A representative lot detected ALK in Immunofluorescence applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

Immunoprecipitation Analysis: A representative lot immunoprecipitated ALK in Immunoprecipitation applications (Pulford, K., et. al. (2002). Br J Haematol. 116(1):135-41; Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

Immunohistochemistry (Paraffin) Analysis: A representative lot detected ALK in Immunohistochemistry applications (Pulford, K., et. al. (1997). Blood. 89(4):1394-404).

Quality

Evaluated by Immunohistochemistry (Paraffin) in human anaplastic large cell lymphoma tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected ALK in human anaplastic large cell lymphoma tissue sections.

Target description

176.44 kDa calculated.

Physical form

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.