MilliporeSigma
All Photos(3)

Documents

MABC1556

Sigma-Aldrich

Anti-NRF2 Antibody, clone 6H11.1

clone 6H11.1, from mouse

Sign Into View Organizational & Contract Pricing

Select a Size

Synonym(s):
NFE2L2, Nuclear factor erythroid 2-related factor 2, NF-E2-related factor 2, NFE2-related factor 2, HEBP1
eCl@ss:
32160702

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6H11.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... NFE2L2(4780)

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
ABE413SAB4501984SAB5700720
antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

6H11.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

mouse, human, rat

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:5000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, immunoprecipitation (IP): 1:50-1:200, western blot: 1:500-1:2000

isotype

IgG1κ

isotype

-

isotype

-

isotype

-

General description

Nuclear factor erythroid 2-related factor 2 (UniProt: Q16236; also known as NF-E2-related factor 2, NFE2-related factor 2, HEBP1) is encoded by the NFE2L2 (also known as NRF2) gene in human. It is widely expressed with high expression in adult muscle, kidney, lung, liver and in fetal muscle. NFE2L2 is a transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes and is essential for the coordinated up-regulation of genes in response to oxidative stress. It contains an acidic activation domain in the N-terminus and DNA binding domain in the C-terminus. NFE2L2 is usually localized in the cytoplasm under unstressed conditions, but translocates to the nucleus when subjected to induction by electrophilic agents. Phosphorylation of Ser40 by protein kinase C in response to oxidative stress dissociates NFE2L2 from its cytoplasmic inhibitor KEAP1, thereby promoting its translocation into the nucleus. Acetylation at Lys596 and Lys599 increases its nuclear localization whereas deacetylation by SIRT1 enhances cytoplasmic presence. NFE2L2 is shown to be down-regulated by ENC1 via a proteasomal ubiquitin-independent protein catabolic process.

Specificity

Clone 6H11.1 detects NRF2 in HepG2 cells. It targets an epitope within 15 amino acids in the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 15 amino acids from the C-terminal region of human Nuclear factor erythroid 2-related factor 2 (NRF2).

Application

Detect NRF2 using this mouse monoclonal Anti-NRF2 Antibody, clone 6H11.1, Cat. No. MABC1556. It has been used in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NRF2 in human liver, human small intestine, human tonsil, and human pancreas.
Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NRF2 in 10 µg of HepG2 cell lysate.

Target description

~64 kDa observed, 67.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Anna Kiefer et al.
Mycotoxin research, 39(3), 303-316 (2023-06-23)
The KEAP1-Nrf2/ARE pathway is a pivotal cytoprotective regulator against oxidative stress which plays an important role in the development of many inflammatory diseases and cancer. Activation of the Nrf2 transcription factor by oxidative stress or electrophiles regulates antioxidant response element

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service