MilliporeSigma
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MABC679

Sigma-Aldrich

Anti-RUNX1 Antibody, clone 2B5

ascites fluid, clone 2B5, from mouse

Synonym(s):
Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2

Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

ascites fluid

antibody product type

primary antibodies

clone

2B5, monoclonal

species reactivity

human

technique(s)

western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

wet ice

Gene Information

human ... RUNX1(861)

General description

Runt-related transcription factor 1 (RUNX1), also known as Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2 (CBF-alpha2), Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B/PEBP2-alpha B), SL3-3 enhancer factor 1 alpha B subunit, and SL3/AKV core-binding factor alpha B subunit is encoded by the human gene named RUNX1/AML1, CBFA2. RUNX1 is a transcription factor that binds to a wide variety of promoters and enhancers belonging to many types of genes from T cell receptor genes to LCK, IL-3 and GM-CSF. Its major normal role is in the development of blood cells and hematopoiesis. It is expressed in most tissues except in normal brain and heart. Levels are highest in the bone marrow, thymus and peripheral lymphocytes. Defects and or translocations involving RUNX1 are the cause for myeloid leukemias as well as lymphoblastic leukemia. Mutations in RUNX1 function are also linked with many breast cancers. Finally, mutations in RUNX1 have recently been shown to be the cause of cancers in epithelial tissues and linked to squamous cell carcinomas. EMD-Millipore’s Anti-RUNX1 mouse monoclonal antibody has been tested in western blot on Jurkat cell lysates and in ELISA using recombinant RUNX1 protein.

Immunogen

Synthesized peptide of human RUNX1.

Application

Anti-RUNX1 Antibody, clone 2B5 is a highly specific mouse monoclonal antibody & that targets RUNX & has been tested in western blotting.
Research Category
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected RUNX1 in Jurkat cell lysate.

Optimal working dilutions must be determined by end user.

Target description

~55 kDa observed. Uncharacterized bands may appear in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Unpurified

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Jurkat cell lysate

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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