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MABC945

Sigma-Aldrich

Anti-TRIM3 Antibody, clone 13D12.1

clone 13D12.1, from mouse

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Synonym(s):
Tripartite motif-containing protein 3, Brain-expressed RING finger protein, RING finger protein 22, RING finger protein 97, TRIM3
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

13D12.1, monoclonal

species reactivity

rat, human, mouse

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TRIM3(10612)

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Anti-TRIM11 Antibody

biological source

mouse

biological source

mouse

biological source

rabbit

biological source

rabbit

clone

13D12.1, monoclonal

clone

3B5.1, monoclonal

clone

polyclonal

clone

polyclonal

Gene Information

human ... TRIM3(10612)

Gene Information

human ... TRIM33(51592)

Gene Information

human ... TRIM11(81559)
mouse ... Trim11(94091)

Gene Information

human ... TRIM31(11074)

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

isotype

IgG1κ

isotype

IgG1κ

isotype

-

isotype

-

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General description

Tripartite motif-containing protein 3 (UniProt O75382; also known as Brain-expressed RING finger protein, RING finger protein 22, Ring finger protein 97, Tripartite motif protein TRIM3, Tripartite motif-containing 3) is encoded by the TRIM3 (also known as BERP, HAC1, RNF22, RNF97) gene (Gene ID 10612) in human. TRIM3 belongs to a family of more than 70 TRIM/RBCC proteins characterized by their shared tripartite motif structure, also known as the RBCC (RING finger, B-box, and coiled-coil) domain. The B-box and coiled coil regions are believed to participate in protein-protein interactions and formation of macromolecular complexes. TRIM proteins are involved in diverse cellular processes, including cell proliferation, differentiation, oncogenesis, and apoptosis. The presence of a RING domain suggests that TRIM proteins function as E3 ubiquitin ligases. TRIM3 is reported to catalyze the transfer of ubiquitin from UbcH5a to p21, a protein necessary for the proliferation of a subset of PDGF (platelet-derived growth factor)-transformed proneural glioma cells. Loss of TRIM3 heterozygosity is found in more than 10% of human GBMs (glioblastomas), while reducing TRIM3 expression facilitates tumor development and accelerates the progression of oligodendroglioma in a mouse model.

Immunogen

GST-tagged recombinant protein corresponding to the internal fragment of human TRIM3.

Application

Anti-TRIM3 Antibody, clone 13D12.1 is an antibody against TRIM3 Antibody for use in Western Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected TRIM3 in mouse brain and human skeletal muscle tissue.

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TRIM3 in 10 µg of HEK293 cell lysate.

Target description

~80 kDa observed. 80.8 kDa (Isoform 1), 79.6 kDa (Isoform 2), 72.1 kDa (Isoform 3), 67.7 kDa (Isoform 4) calculated.

Physical form

Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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