MilliporeSigma
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MABC95

Sigma-Aldrich

Anti-S1PR2/EDG5 Antibody, clone 6E8.1

clone 6E8.1, from mouse

Synonym(s):
Sphingosine 1-phosphate receptor 2, S1P receptor 2, S1P2, Endothelial differentiation G-protein coupled receptor 5, Sphingosine 1-phosphate receptor Edg-5, S1P receptor Edg-5
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

6E8.1, monoclonal

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgMκ

NCBI accession no.

UniProt accession no.

shipped in

wet ice

Gene Information

human ... S1PR2(9294)

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biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

clone

6E8.1, monoclonal

clone

SuGr-20 18A9, monoclonal

clone

16B9.1, monoclonal

clone

5.5, monoclonal

species reactivity

human

species reactivity

human

species reactivity

rat, human

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgMκ

isotype

IgG2bκ

isotype

IgMκ

isotype

IgG1κ

General description

Sphingosine 1-phosphate receptor 2 (S1PR2), otherwise known as EDG5, is a ubiquitous G-protein coupled receptor for lysosphingolipid sphingosine 1-phosphate (S1P). It is coupled to Gi, G12/13, and Gq pathways to regulate a number of second messengers and effector molecules, including cAMP, PLC, MAPK, RhoA, and Rac. S1PR2 mediates the effects of S1P on cell motility and embryonic development, among other cellular processes.

Specificity

This antibody recognizes the cytoplasmic domain of S1PR2/EDG5.

Immunogen

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human S1PR2/EDG5.

Application

Anti-S1PR2/EDG5 Antibody, clone 6E8.1 detects level of S1PR2/EDG5 & has been published & validated for use in Western Blotting, IHC(P).
Immunohistochemistry Analysis: A representative lot detected S1PR2/EDG5 in human tonsil, liver, colorectal cancer tissues.

Quality

Evaluated by Western Blot in human liver tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected S1PR2/EDG5 in 10 mg of human liver tissue lysate.

Target description

~35 kDa observed

Physical form

Format: Purified

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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