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MABE1789

Sigma-Aldrich

Anti-APC11 Antibody, clone CRQ1.1

clone CRQ1.1, from mouse

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Synonym(s):
Anaphase-promoting complex subunit 11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein
eCl@ss:
32160702

biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

CRQ1.1, monoclonal

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), monkey (based on 100% sequence homology), bovine (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

technique(s)

western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... ANAPC11(51529)

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Anti-APC10 Antibody from rabbit, purified by affinity chromatography

Sigma-Aldrich

ABE608

Anti-APC10 Antibody

antibody form

purified antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

CRQ1.1, monoclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

mouse

species reactivity

human, mouse

packaging

antibody small pack of 25 μg

packaging

antibody small pack of 25 μL

packaging

-

packaging

-

technique(s)

western blot: suitable

technique(s)

immunoblotting: 0.04-0.4 μg/mL, immunofluorescence: 0.25-2 μg/mL, immunohistochemistry: 1:50-1:200

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: 1:40000, immunohistochemistry: 1:50-1:100

General description

Anaphase-promoting complex subunit 11 (UniProt: Q9NYG5; also known as APC11, Cyclosome subunit 11, Hepatocellular carcinoma-associated RING finger protein) is encoded by the ANAPC11 (also known as HSPC214) gene (Gene ID: 51529) in human. APC11 is one of the components of the anaphase-promoting complex (APC) that belongs to the RING-box family of proteins. APC is a large E3 ubiquitin ligase that responsible for targeting proteins for degradation during mitosis. The APC is assembled from 13 different proteins where in APC2 binds to APC11 forming the catalytic core that recruits the E2 ubiquitin conjugating enzyme. APC11 has with multiple zinc -binding sites with a RING-type zinc-finger domain (aa 34-77). It is expressed at high levels in the skeletal muscle and heart and in moderate levels in brain, kidney, and liver. Two isoforms of APC11 have been described that are produced by alternative splicing. (Ref.: Schreiber, A et al., (2011). Nature. 470(7333); 227-32).

Specificity

Clone CRQ1.1 specifically detects human Anaphase-promoting complex subunit 11 (APC11). It targets an epitope with in 9 amino acids from the C-terminal region.

Immunogen

A linear peptide corresponding to 9 amino acids from the C-terminal region of human Anaphase-promoting complex subunit 11 (APC11).
Epitope: C-terminus

Application

Anti-APC11, clone CRQ1.1, Cat. No. MABE1789, is a highly specific mouse monoclonal antibody that targets Anaphase-promoting complex subunit 11 and has been tested in Western Blotting.
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: A representative lot detected APC11 in Western Blotting applications (Sedgwick, G.G., et. al. (2013). EMBO J. 32(2):303-14).

Western Blotting Analysis: A representative lot detected APC11 in APC11-Flag expressed in HeLa cells following tetracycline treatment.

Quality

Isotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG2a.

Target description

9.84 kDa calculated.

Physical form

Format: Purified
Protein G purified
Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Meenakshi Vij et al.
Neuro-oncology advances, 5(1), vdad069-vdad069 (2023-06-16)
Isocitrate dehydrogenase (IDH) mutations are thought to represent an early oncogenic event in glioma evolution, found with high penetrance across tumor cells; however, in rare cases, IDH mutation may exist only in a small subset of the total tumor cells

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