MABE1828

Anti-beta-Catenin Antibody, clone mAb-Cat1

clone mAb-Cat1, from rat

• Synonym(s): beta-Catenin 1, Catenin beta-1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: mAb-Cat1, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mouse (based on 100% sequence homology), bovine (based on 100% sequence homology)
• packaging: antibody small pack of 25 μL
• technique(s): ChIP: suitable; dot blot: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_001091679.1
• UniProt accession no.: P35222
• target post-translational modification: unmodified
• Gene Information: human ... CTNNB1(1499) ; mouse ... Ctnnb1(12387)

Description

General description

Catenin beta-1 (UniProt: P35222; also known as beta-Catenin) is encoded by the CTNNB1 (also known as CTNNB, OK/SW-cl.35, PRO2286) gene (Gene ID: 1499) in human. Catenin beta-1 is a key downstream component of the canonical Wnt signaling pathway that participates in intercellular adhesion and Wnt-mediated transcriptional activation. In the absence of Wnt, it forms a complex with AXIN1, AXIN2, APC, CSNK1A1, and GSK-3 beta that promotes phosphorylation on N-terminal serine and threonine residues that leads to its subsequent degradation by the proteasome. In the presence of Wnt ligand, it is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, which leads to activation of Wnt responsive genes. O-glycosylation at serine 23 is reported to decreases its nuclear localization and transcriptional activity. Wnt/beta-catenin signaling is shown to be essential for embryonic stem cell (ESC) pluripotency and differentiation. In ESC beta-catenin is trimethylated on Lysine 49 by Ezh2 methyltransferase and this trimethylated form represses neuronal differentiation genes Sox1 and Sox3. Mutations in CTNNB1 gene are shown to cause colorectal cancers, medulloblastoma, and ovarian cancers. (Ref.: Hoffmeyer, K., et al. (2017). Cell Reports 18(12); 2815-2824.

~100 kDa observed; 85.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunogen

BSA-conjugated linear peptide corresponding to 19 amino acids from the N-terminal region of human beta-catenin 1.

Application

Anti-beta-Catenin, clone mAb-Cat1, Cat. No. MABE1828, is a highly specific rat monoclonal antibody that targets beta-catenin 1 and has been tested for use in Chromatin Immunoprecipitation (ChIP), Dot Blot, Immunoprecipitation, and Western Blottiing.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: A representative lot detected beta-Catenin in Western Blotting applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Immunoprecipitation Analysis: A representative lot immunoprecipitated beta-Catenin in Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Dot Blot Analysis: A representative lot detected beta-Catenin in Dot Blot applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot immunoprecipitated beta-Catenin in Chromatin Immunoprecipitation applications (Hoffmeyer, K., et. al. (2017). Cell Rep. 18(12):2815-2824).

Biochem/physiol Actions

This rat monoclonal antibody detects beta-catenin 1 unmodified and methylated or acetylated on lysine 49. It targets an epitope with in 19 amino acids from the N-terminal region.

Physical form

Format: Purified

Protein G purified

Purified rat monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Western Blotting in SW 480 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected beta-Catenin in SW 480 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.