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MABE2033

Sigma-Aldrich

Anti-GINS1 Antibody, clone 192B

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Synonym(s):
DNA replication complex GINS protein PSF1;GINS complex subunit 1

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

192B, monoclonal

mol wt

calculated mol wt 22.99 kDa
observed mol wt ~23 kDa

purified by

using protein G

species reactivity

human

packaging

antibody small pack of 100 μg

technique(s)

immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG1κ

epitope sequence

Unknown

Protein ID accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... CD163(9332)

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vibrant-m

MABE2033

Anti-GINS1 Antibody, clone 192B

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

biological source

mouse

biological source

rat

biological source

mouse

biological source

rabbit

species reactivity

human

species reactivity

human, mouse

species reactivity

human

species reactivity

human

UniProt accession no.

Q14691

UniProt accession no.

Q9D3E6

UniProt accession no.

Q14691

UniProt accession no.

Q14691

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General description

DNA replication complex GINS protein PSF1 (UniProt: Q14691; also known as GINS complex subunit 1) is encoded by the GINS1 (also known as KIAA0186, PSF1) gene (Gene ID: 9837) in human. GINS1 is a component of the GINS (Go-Ichi-Ni-San) complex, a heterotetramer of GINS1, 2, 3, and 4, that plays an essential role in the initiation of DNA replication and progression of DNA replication forks. GINS complex is highly conserved in mammalian species. Its each subunit is composed of two types of structural domains: an -helix rich (A) domain and a β-strand rich (B) domain. GINS1 is highly expressed in undifferentiated cells and its deficiency in mice is shown to cause embryonic lethality by day 6.5. Its levels are significantly increased in several types of cancers, including lung, breast, and prostate cancers and several cancer cell lines. Mutations in GINS1 gene are known to cause immunodeficiency 5 (IMD55) that is characterized by chronic neutropenia, natural killer cell deficiency, and recurrent viral and bacterial infections, and most patients exhibit growth retardation. (Ref.: Hsieh, H-Y., et al. (2019). Cancer Sci. 111(7); 2400-2412; Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7); 2087-2095).

Specificity

Clone 192B is a mouse monoclonal antibody that detects Human DNA replication complex GINS protein PSF1 (GINS1).

Immunogen

Full-length recombinant human DNA replication complex GINS protein PSF1 (GINS1).

Application

Quality Control Testing

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GINS1 in HEK293 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected GINS1 in MOLT-4 and Daudi cell lysates.

Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated GINS1 in Immunoprecipitation applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Immunohistochemistry Applications: A representative lot detected GINS1 in Immunohistochemistry applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Western Blotting Analysis: A representative lot detected GINS1 in Western Blotting applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Immunofluorescence Analysis: A representative lot detected GINS1 in Immunofluorescence applications (Aparicio, T., et al. (2009). Nucleic Acids Res. 37(7): 2087-95).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-GINS1, clone 192B, Cat. No. MABE2033, is a mouse monoclonal antibody that detects GINS complex subunit 1 and is tested for use in Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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