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MABE2037

Sigma-Aldrich

Anti-STAG1/SA1 Antibody, clone SUSI63B

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Synonym(s):
Cohesin subunit SA-1 (UniProt: Q9D3E6;also known as SCC3 homolog 1;Stromal antigen 1

biological source

rat

Quality Level

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

SUSI63B, monoclonal

mol wt

calculated mol wt 144.44 kDa
observed mol wt ~145 kDa

purified by

using protein G

species reactivity

human, mouse

packaging

antibody small pack of 100 μL

technique(s)

flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG2aκ

epitope sequence

N-terminal

Protein ID accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

mouse ... Pnpla3(116939)

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Anti-SRF Antibody, clone 2C5

clone

SUSI63B, monoclonal

clone

6G3-G6, monoclonal

clone

2C5, monoclonal

clone

2E9, monoclonal

biological source

rat

biological source

mouse

biological source

rat

biological source

mouse

species reactivity

human, mouse

species reactivity

SARS coronavirus

species reactivity

human, mouse

species reactivity

human

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

Gene Information

mouse ... Pnpla3(116939)

Gene Information

human ... GINS1(9837)

Gene Information

mouse ... Srf(20807)

Gene Information

human ... STAG1(10274)

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General description

Cohesin subunit SA-1 (UniProt: Q9D3E6; also known as SCC3 homolog 1, Stromal antigen 1) is encoded by the Stag1 (also known as Sa1) gene (Gene ID: 20842) in murine species. Cohesin are known to mediate sister chromatid cohesion and 3D genome folding. They hold the sister chromatids together to ensure correct DNA repair by homologous recombination and proper chromosome segregation during cell division. They are essential for cell proliferation. The cohesin complex forms a large proteinaceous ring within which sister chromatids can be trapped and at anaphase stage. The complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. This complex may also play a role in spindle pole assembly during mitosis. Two variant cohesin complexes containing Structural Maintenance of Chromosomes (SMC) 1, SMC3, RAD21 and either STAG/SA1 or STAG2/SA2 are present in all cell types. Before prophase, STAG1/SA1 appears to be scattered along chromosome arms. However, during prophase, most of cohesin complexes dissociate from chromatin. This dissociation is probably due to its phosphorylation by PLK1. At anaphase, the RAD21 subunit is cleaved that leads to the dissociation of the complex from chromosomes, allowing their separation. (Ref.: De Koninck, M., et al. (2020). Cell Rep.32(6); 108014; Kojic, A., et al. (2018). Nat. Struct. Mol. Biol. 25(6); 496-504).

Specificity

Clone SUSI63B is a rat monoclonal antibody that detects STAG1/SA1. It targets an epitope within the N-terminal region.

Immunogen

MBP-tagged recombinant fragment corresponding to the first 225 amino acids from N-terminal region of mouse Cohesin subunit SA-1 (STAG1).

Application

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected STAG1/SA1 in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected STAG1/SA1 in HeLa and RAW264.7 cell lysates.

Flow Cytometry Analysis: A representative lot detected STAG1/SA1 in Flow Cytometry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Immunofluorescence Analysis: A representative lot detected STAG1/SA1 in Immunofluorescence applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Western Blotting Analysis: A representative lot detected STAG1/SA1 in Western Blotting applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014; Kojic, A., et al. (2018). Nat Struct Mol Biol. 25(6): 496-504; Cuadrado, A., et al. (2019). Cell Rep. 27(12): 3500-3510.e4).

Immunohistochemistry Applications: A representative lot detected Stag1 in Immunohistochemistry applications (De Koninck, M. et al. (2020). Cell Rep. 32(6): 108014).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-STAG1/SA1, clone SUSI63B, Cat. No. MABE2037, is a rat monoclonal antibody that detects STAG1/SA1 and is tested for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, and Western Blotting.

Physical form

Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Storage and Stability

Recommended storage: +2°C to +8°C.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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