MABF1466
Anti-CD45 (Mouse), clone 30-F11 Antibody
clone 30-F11, from rat
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Synonym(s):
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen (L-CA), Lymphocyte antigen 5 (Ly-5), T200, CD45, CD45
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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biological source
rat
Quality Level
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
30-F11, monoclonal
species reactivity
mouse
technique(s)
flow cytometry: suitable
isotype
IgG2bκ
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
mouse ... Ptprc(19264)
Related Categories
General description
The 30-F11 antibody reacts with mouse CD45, which is one of the most abundant hematopoietic markers and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others. The 30-F11 antibody is widely used as a leukocyte marker for B cells, T cell subsets and NK cell subsets.
Immunogen
Immunogen information for this antibody is proprietary information.
Application
Research Category
Inflammation & Immunology
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
Immunoglobulins & Immunology
This rat monoclonal Anti-CD45 Antibody (Mouse), clone 30-F11, Cat. No. MABF1466 is validated for use in Flow Cytometry, for the detection of CD45.
Quality
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD45 in one million C57Bl/6 splenocytes.
Flow Cytometry Analysis: 0.125 μg from a representative lot detected CD45 in one million C57Bl/6 splenocytes.
Physical form
Affinity Chromatography
Format: Purified
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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