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MABF154C

Sigma-Aldrich

Anti-CD8a (Mouse), PE-Cy7, clone 53-6.7 Antibody

clone 53-6.7, from rat, PE-Cy7

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Synonym(s):
T-cell surface glycoprotein CD8 alpha chain, T-cell surface glycoprotein Lyt-2, CD8a, CD8a
eCl@ss:
32160702
NACRES:
NA.44

biological source

rat

Quality Level

conjugate

PE-Cy7

antibody form

purified antibody

antibody product type

primary antibodies

clone

53-6.7, monoclonal

species reactivity

mouse

packaging

antibody small pack of 25 μg

technique(s)

flow cytometry: suitable

isotype

IgG2aκ

UniProt accession no.

target post-translational modification

unmodified

Gene Information

mouse ... Cd8A(12525)

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MABF154BMABF153DMABF153E
clone

53-6.7, monoclonal

clone

53-6.7, monoclonal

clone

53-6.7, monoclonal

clone

53-6.7, monoclonal

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

Gene Information

mouse ... Cd8A(12525)

Gene Information

mouse ... Cd8A(12525)

Gene Information

mouse ... Cd8A(12525)

Gene Information

mouse ... Cd8A(12525)

biological source

rat

biological source

rat

biological source

rat

biological source

rat

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

species reactivity

mouse

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General description

The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD8a Antibody (Mouse), PE-Cy7, clone 53-6.7, Cat. No. MABF1546 is validated for use in Flow Cytometry, for the detection of CD8a.

Quality

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD8a in one million C57Bl/6 splenocytes.

Physical form

Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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